Positive Impact of Ethyl Acetate Fraction of Kalanchoe pinnata on Anti-Smith Antibody and T Reg in Lupus Mice
| Main Authors: | INDRIYANTI, NIKEN, KHOTIB, JUNAIDI, SOEROSO, JOEWONO |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2017
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/454 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/454/302 |
Daftar Isi:
- Kalanchoe pinnata (Lmk) Pers is a medicinal plant that has many activities, such as antioxidant, anti-infl ammation and immunosuppressant activities. Based on our previous study, aqueous extract of this plant leaves has a repairing eff ect on lupus nephritis model. The expected active compounds are flavonoids. Those compounds are separated in the ethyl acetate fraction. Therefore, this study observed the activity of the ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers on reducing the level of Anti- Smith autoantibody and the regulation function of CD4+CD25+ T reg in lupus mice. The experimental groups were a negative control group that received placebo, ethyl acetate fraction of Kalanchoe pinnata (Lmk) Pers (EF-KP) group and a positive control group that received cyclophosphamide. The anti-Sm level was measured by using indirect ELISA. The spleen cells were prepared for CD4+CD25+ T reg assay using fl ow cytometry. The result of this experiment was a reduction of anti-Sm antibody level. The decrease was suffi cient to stabilize lupus condition. The fl ow cytometry assay results showed the increase in the relative percentage of CD4+CD25+ T reg. This function can inhibit the reactivity of autoantigens, so it prevents tissue and organ damage from lupus. However, further research is needed to observe the complete mechanism.
- Kalanchoe pinnata (Lmk) Pers memiliki banyak aktivitas, seperti antioksidan, antiinfl amasi, dan imunosupresan. Berdasarkan penelitian sebelumnya, ekstrak air daun Kalanchoe pinnata (Lmk) Pers memiliki efek memperbaiki struktur dan fungsi ginjal pada model lupus nefritis. Senyawa aktif yang diduga adalah senyawa-senyawa golongan fl avonoid yang masuk ke dalam fraksi etil asetat sesuai metode fraksinasi Cruz(1). Tujuan penelitian ini adalah membuktikan aktivitas fraksi etil asetat Kalanchoe pinnata (Lmk) Pers (EF-KP) untuk menurunkan kadar antibodi Anti-Smith dan mengatur fungsi regulasi sel T regulator CD4+CD25+. Kelompok uji terdiri dari kontrol negatif (placebo), FE-KP, dan kontrol positif (siklofosfamid). Kadar anti-Sm diukur menggunakan indirect ELISA. Selanjutnya sel-sel limpa diisolasi untuk pengukuran sel T reg CD4+CD25+ dengan metode fl ow cytometry. Hasil penelitian menunjukkkan bahwa ada penurunan rata-rata kadar anti-Sm yang cukup untuk menstabilkan kondisi model lupus. Hasil pengukuran T reg menunjukkan peningkatan jumlah marker sel T reg CD4+CD25+. Peningkatan fungsi regulasi ini mampu menghambat reaktivitas autoantigen, sehingga mencegah kerusakan jaringan dan organ pada penyakit lupus. Namun, penelitian selanjutnya perlu dilakukan untuk mengamati mekanisme kerja FE-KP pada biomarker lupus yang lain.