Genotyping Study of Cytochrome P450 2A6 Alel CYP2A6*1 and CYP2A6*9 among Javanese Indonesian Smokers
| Main Authors: | PATRAMURTI, CHRISTINE, FENTY, FENTY |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2017
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/453 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/453/301 |
Daftar Isi:
- CYP2A6 belongs to the CYP2 family of P450 cytochromes were highly polimorphy. CYP2A6*1 (wild type) was an active allele, CYP2A6*9 was agen decreased allele and the CYP2A6*4 was an inactive allele. By using the polymerase chain reaction technique (PCR), the CYP2A6 polymorphism was studied among Javanese Indonesian smokers. In the process of genotyping the CYP2A6*9 allele, some subjects who had been genotyped as CYP2A6*1/*4 in our previous studies were regenotyped as CYP2A6*9 in this study. The primer forward 2A6*9S and the primer reverse 2A6*9AS-wild type were used in these study. The Promega Go Taq Green Master Mix reagent were used to amplifl y the allele CYP2A6*1 in the positions at -395 to -28 of the CYP2A6 gene. The sample studied consisted of 20 smokers with Cigarrete per-Day (CPD) <10 and 13 smoker with CPD 11-20 from Javanese Indonesian population. In theses research, the subjects had been genotyped as CYP2A6*1/*4 in our previous studies. The allele frequencies of CYP2A6*1, CYP2A6*4, and CYP2A6*9 were 48.5%, 48.5%, and 3%, respectively. When these allele were considered simultaneously, among the subject, 63.9% were genotyped for CYP2A6*1/*4 and 6.1% were genotyped for CYP2A6*1/*4/*9. Based on the data collected, it could be concluded that the polymorphism of CYP2A6 among Javanese population sample study was not aff ected on smoking behavior.
- CYP2A6 merupakan salah satu enzim golongan sitokrom P450 yang memiliki bentuk polimorfi . Bentuk aktif enzim ini adalah CYP2A6*1, sedangkan bentuk alel CYP2A6*9 merupakan enzim yang memiliki penurunan aktivitas dan bentuk tidak aktif enzim ini adalah CYP2A6*4. Adanya bentuk polimorfi enzim ini dapat dideteksi menggunakan metode polymerase chain reaction technique (PCR). Pada penelitian ini, identifi kasi CYP2A6*9 dilakukan terhadap subjek uji yang pernah terlibat pada penelitian terdahulu dan telah teridentifi kasi memiliki genotipe CYP2A6*1/*4. Primer yang digunakan pada penelitian ini adalah primer forward 2A6*9S dan primer reverse 2A6*9AS-wild type. Proses amplifi kasi DNA pada posisi -395 sampai -28 dari gen CYP2A6 dilakukan menggunakan enzim Promega Go Taq Green Master Mix. Subjek uji berasal dari suku Jawa Indonesia yang terdiri dari 20 orang perokok dengan Cigarrete per-Day (CPD) <10 dan 13 smoker dengan CPD 11-20. Hasil penelitian menunjukkan frekuensi alel CYP2A6*1, CYP2A6*4, and CYP2A6*9 berturut-turut sebesar 48,5%; 48,5%; dan 3%. Bentuk genotipe CYP2A6*1/*4 diantara subjek uji sebesar 63,9%, sedangkan genotipe CYP2A6*1/*4/*9 sebesar 6,1%. Berdasarkan hasil penelitian ini, maka dapat disimpulkan bahwa adanya bentuk polimorfi CYP2A6 diantara subjek uji bersuku Jawa Indonesia yang diteliti tidak mempengaruhi perilaku merokok subjek uji.