Isolation and Characterization of Antibacterial Compound Against Streptococcus mutans from Ethanol Extract of Kalanchoe pinnata (Lamk.) Pers. Leavcs
| Main Authors: | YANTIH, NOVI, MARGARET, LISIA, KARTININGSIH, KARTININGSIH |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2012
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/282 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/282/202 |
Daftar Isi:
- Streptococcus mutans is one of the microbes which is responsible for the cause of bad breath. The activity of Kalanchoe pinnata (Lamk.) Pers. leaves ethanol extract is four times stronger than the water extract in inhibiting the growth of Streptococcus mutans. The aim of research is to isolate and characterize an antibacterial compound of ethanol extract of Kalanchoe pinnata (Lamk.) Pers. leaves. The extract was fractionated using column chromatography. The fractions were purified by TLC and the isolate were tested for its purity using HPLC. Isolate was characterized using UV-Vis spectrophotometry and FTIR. Fractionation by column chromatography generated 9 fractions. The fraction VII have the highest diameter of inhibitory against Streptococcus mutans. The yield of isolate was 9.18% of the fraction VII or 0.84% of the ethanol extract with purity of 95%. Isolate was suspected as a group of saponin compound.
- Streptococcus mutans adalah salah mikroba yang bertanggung jawab terhadap bau mulut. Aktivitas ekstrak etanol dari daun Sosor Bebek (Kalanchoe pinnata (Lamk.) Pers.) memiliki kekuatan empat kali dari ekstrak airnya dalam menghambat pertumbuhan Streptococcus mutans. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengkarakterisasi suatu senyawa antibakteri dari ekstrak etanol daun Kalanchoe pinnata (Lamk.) Pers. Ekstrak difraksinasi menggunakan kromatografr kolom. Fraksi dipurifikasi dengan KLT dan diuji kemurnian isolat secara KCKT. Isolat dikarakterisasi menggunakan spektrofotometri UV-Vis dan FTIR. Fraksinasi dcngan kolom kromatograli menghasilkan 9 fraksi. Fraksi VII memiliki DDH terhadap Streptococcus mutans paling besar. Rendemen isolat sebesar 9,18% terhadap fraksi VII atau 0,84% terhadap ekstrak etanol dengan kemurnian 95%. Isolat diduga adalah golongan senyawa saponin.