Production of Beta-Glucan from Saccharomyces cerevisiae and Antioxidant Activity on t-BHP Induced Red Blood Cells
| Main Authors: | KUSMIATI, KUSMIATI, AGUSTINI, NI WAYAN S., TAMAT, SWASONO R., RIANTO, BAYU |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2012
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/276 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/276/198 |
Daftar Isi:
- Beta glucan is a polysaccharide that can be found in cereals, yeast, bacteria, and algae. Beta glucan produced by Saccharomyces cerevisiae has the ability to enhance the body’s defense system. The study aims to investigate the antioxidant activity of B glucan produced by three strains of Saccharomyces sp BR 1, BR 2,and SC. Antioxidant activity assay was carried out in vitro on t BHP induced red blood cells (REC) by observing the level of catalase activity, SOD activity and MDA levels as lipid peroxidation product. The investigation was performed on 6 groups: normal control, negative control, positive control (RBC+ t-BHP + vitamin E 100 IU), and samples (RBC+t-BHP) treated with 1.4 μg/mL β-glucan from Saccharomyces strain BR1, BR2 and SC respectively. Saccharomyces cerevisiae strain SC produced the highest β-glucan of 88.70% (glucose equivalent), while the BR1 and BR2 of 22.56% and 46.28% respectively. Antioxidant activity assay showed that the highest increase in catalase activity (581.48%) was given by the B glucan from SC strain, the highest increase of SOD activity and decrease of MDA levels were given by the β- glucan from BR1 strain (359.17% and 48.18%) as compared to negative controls. Statistical test (α = 0.05) showed antioxidant activity of β-glucan from Saccharomyces Sp BR2 and SC did not differ significantly from the activity of vitamin E (100 IU).
- Beta glukan merupakan polisakarida yang dapat ditemukan pada sereal, khamir, bakteri, dan alga. Beta glukan yang dihasilkan oleh Saccharomyces cerevisiae memiliki kemampuan meningkatkan sistem pertahanan tubuh. Penelitian ini bertujuan untuk menguji aktivitas antioksidan β-glukan yang dihasilkan oleh tiga galur Saccharomyces .sp yaitu BRl , BR2, dan SC. Uji aktivitas antioksidan dilakukan secara in vitro pada sel darah merah (SDM) yang diinduksi t BHP dengan cara mengamati tingkat aktifitas katalase, aktiiitas SOD, dan kadar MDA hasil peroksidasi lipid. Percobaan dilakukan pada 6 kelompok, yaitu: kontrol SDM normal, kontrol negatif, kontrol positif (+ vitamin E 100 IU), dan perlakuan dengan 1,4 μg/mL β-glukan dari masing masing Saccharomyces Sp BR1, BR2, dan SC. Saccharomyces sp galur SC ternyata menghasilkan β-glukan tertinggi sebesar 88,70% (ekivalensi glukosa), sedangkan galur BR1 dan BR2 masing masing sebesar 22,56% dan 46,28%. Basil uji aktivitas antioksidan menunjukkan bahwa peningkatan aktiiitas katalase tertinggi (581,48%) diberikan oleh B glukan dari S. cerevisiae SC, peningkatan aktifitas SOD dan penurunan kadar MDA tertinggi diberikan oleh β-glukan dari Saccharomyces Sp BR1 (359,17% dan 48,18%) dibandingkan dengan kontrol negatif. Uji statistik ((α = 0,05) menunjukkan aktifitas antioksidan β-glukan Saccharomyces Sp BR2 dan SC tidak berbeda nyata dengan aktifitas Vitamin E (100 IU).