Potential of Ethanolic Extract from Leaf of Red Betel Vine (Piper crocatum) as Antimicrobacterium
| Main Authors: | RACHMAWATY, FARIDA JULIANTINA, HISYAM, BARMAWI, SOESATYO, MARSETYAWAN HNE, WIBAWA, TRI |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2013
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/241 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/241/170 |
Daftar Isi:
- : The incidence of tuberculosis in Indonesia is high. There is a tendency for some people to use herbs for the treatment of tuberculosis. This research aims to investigate the potency of antimycobacterium property of red betel Vine (Piper crocatum) ethanolic extract against Mycobacterium tuberculosis (M. tuberculosis). The red betel vine leaf was extracted using standard ethanol solvent. The extract was tested on Mycobacterium tuberculosis H3 7Rv. The experiment was conducted by using serial dilution method with 50%, 40%, 25%, 20%, 12.5%, 10%, and 6.25% red betel vine ethanolic extract in Middlebrook 7H9 medium and subseqently inoculated into Lowenstein Jensen (LJ) medium. The result was analyzed descriptively by observing the minimum bactericidal concentration (MBC). The experiment was repeated three times to obtain consistent result. There is no bacterial growth in LJ media at concentrations of 50%, 40%, 25%, 20%, 12.5%, and 10% of red betel vine ethanolic extract. The bacteria was able to grow only at the concentration of 6.25% red betel vine ethanolic extract. Thus the ethanolic extract of red betel vine (Piper crocatum) indicates an antimycobaterium activity against M. tuberculosis H37Rv. The MBC is found at the concentration of 10%.
- Insidensi penyakit tuberkulosis di Indonesia masih tinggi. Ada kecenderungan pada sebagian masyarakat menggunakan tanaman herbal untuk pengobatan 'tuberkulosis. Penelitian ini bertujuan untuk mengkaji potensi antimikobakterium ekstrak etanol daun sirih merah (Piper crocatum) terhadap Mycochterium tuberculosis (M tuberculosis). Daun sirih merah dibuat ekstrak dengan pelarut etanol secara maserasi. Ekstrak yang telah dihilangkan pelarutnya tersebut diujikan pada bakteri M. tuberculosis H37Rv. Metode uji dilakukan secara dilusi serial dengan konsentrasi 50%, 40%, 25%, 20%, 12.5%, 10%, dan 6.25% pada media Middlebrook 7H9 dan dikultur pada media Lowenstein Jensen. Hasil dianalisis secara deskriptif dengan melihat Kadar Bunuh Minimal (KBM). Percobaan dilakukan pengulangan 3 kali hasil konsisten. Hasil penelitian menunjukkan bahwa tidak ada pertumbuhan bakteri pada media Lowenstein Jensen pada konsentrasi 50%, 40%, 25%, 20%, 12.5%, dan 10%. Pertumbuhan bakteri hanya terdapat pada konsentrasi 6,25%. Dengan demikian ekstrak etanol daun sirih merah (Piper crocatum) mempunyai efek antimikobakterium terhadap M. tuberculosis H3 7Rv dengan Kadar Bunuh Minimalnya pada konsentrasi 10%.