FREE RADICAL SCAVENGING ACTIVITY AND ANTIHYPERGLYCEMIC POTENCY OF VARIOUS GAMBIER COLLECTED FROM SEVERAL INDONESIAN MARKETS
| Main Authors: | WIDIYARTI, GALUH, SUNDOWO, ANDINI |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2012
|
| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/233 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/233/166 |
Daftar Isi:
- Gambier (uncaria gambier) is known to have antioxidant properties and some studies attributed to the presence of polyphenols. The objective of this study was to investigate the potential of various gambier available in Indonesian market as free radical scavenger and to evaluate its antihyperglycemic potency as α-glucosidase inhibator. Isolation of catechin was carried out by extraction with distilled ethyl acetate as solvent. Analysis of catechin in the gambier dried extracts were carried out by TLC method. The molecular weight and catechin content of gambier dried extracts were determined by analyzing mass spectra and spectrophotometry, respectively. The free radical scavenging aactivity ofextracts were measured by using 1, 1-diphenyl1-2-picrylhydrazyl (DPPH) as stable free radical compound. The antihyperglycemic potency of gambier dried extracts were asseyed, as α-glucosidase inhibator. The results showed that various gambier available in the market were very active as antioxidant, indicated by the IC50 vvalues of gambier dried extracts were 4.6 to 18.2 μg/mL. Whilst th IC50 for glucosidase inhibition varies from 40.45 to 52.43 μg/mL, and classified as a moderate antidiabetic.
- Gambir (Uncaria gambier) telah diketahui mempunyai aktivitas antioksidan karena mengandung polifenol. Penelitian ini bertujuan untuk mengetahui aktivitas penangkapan radikal bebas dan potensi antihiperglikemik sebagai inhibator α-glukosidase dari gambir yang dikoleksi dari beberapa pasar Indonesia. Isolasi katekin dilakukan dengan cara ekstraksi dengan menggunakan pelarut etil asetat. Analisis awal katekin yang terkandung dalam ektrak kering gambir dilakukan dengan KLT. Evaluasi berat molekul dan kandungan katekin pada ekstrak kering gambir dilakukan masing-masing dengan spektroskopi massa dan spektrofotometer. Aktivitas penangkapan radikal bebas diukur dengan menggunakan 1, 1-difenil-2-pikrilhidrazil (DPPH) sebagai sumber radikal bebas. Potensi antihiperglikemik ekstrak kering gambir dianalisis sebaga inhibator α-glukosidase. Hasil penelitian menunjukan bahwa beberapa gambir yang ada di pasar Indonesia sangat aktif sebagai antioksidan, yang ditunjukkan dengan nilai IC50 dari 4,6 sampai 18,2 μg/mL. Nilai IC50 untuk inhibisi α-glukosidase bervariasi antara 40,45 sampai 52,43 μg/mL, dan diklasifikasikan sebagai antioksidan moderat.