The Effect of Rambutan Seed (Nephelium lappaceum L.) Infusion on Blood Glucose and Pancreas Histology of Mice Induced with Alloxan
| Main Authors: | RAHAYU, LESTARI, ZAKIR, LATIF, KEBAN, SESILIA ANDRIANI |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2013
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/228 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/228/162 |
Daftar Isi:
- Rambutan (Nephelium lappaceum L.) is a plant which can be used to treat various diseases, including diabetes mellitus. This research was conducted to investigate the effect of rambutan seed infusion on the mice blood glucose levels and body weight. In this research, 30 mice (Mus musculus) were divided into 6 groups, each group consists of 5 mice. The 6 groups consists of a normal control group (distilled water), negative control group (alloxan tetrahydrate 250 mg/kg bw + distilled water), three experimental groups (induced with alloxan 250 mg/kg bw) were given rambutan seed infusion of 1.56 gram/kg bw, 2.34 gram/kg bw and 3.12 gram/kg bw and a positive control group (alloxan tetrahydrate 250 mg/kg bw + treated with glibenclamide 0.65 mg/kg bw). After given treatment for 16 days, it was observed that high dose of rambutan seed infusion has significant effects in reducing the blood glucose and body weight of mice. Histology of the pancreas were checked and the ammount of live pancreatic beta cells found in high dose of rambutan seed infusion were almost the same as the positive control group. It can be concluded that the effect of high dose of rambutan seed infusion (3.12 gram/kg bw) was not significantly different from glibenclamide (0,65 mg/kg bw).
- Rambutan (Nephelium lappaceum L.) merupakan salah satu tanaman yang dapat digunakan untuk mengobati berbagai macam penyakit, salah satunya diabetes melitus. Penelitian ini dilakukan untuk menguji efek seduhan biji rambutan terhadap penurunan kadar glukosa darah dan berat badan mencit. Dalam penelitian ini 30 mencit (Mus musculus) dibagi menjadi 6 kelompok perlakuan, masing-masing kelompok terdiri dari 5 ekor. Keenam kelompok tersebut adalah kontrol normal (air suling), kontrol negatif (aloksan 250 mg/kg bb + air suling), tiga kelompok uji (diinduksi dengan aloksan tetrahidrat 250 mg/kg bb) kemudian masing-masing kelompok diberi air seduhan biji rambutan dosis 1,56 g/kg bb, 2,34 g/kg bb dan 3,12 gram/kg bb) dan kontrol positif (diinduksi aloksan tetrahidrat 250 mg/kg bb + glibenklamida 0,65 mg/kg bb). Setelah diberikan perlakuan selama 16 hari diperoleh hasil bahwa pemberian air seduhan biji rambutan pada dosis tinggi mampu menurunkan kadar glukosa darah dan berat badan secara signifikan. Pemeriksaan histologi pankreas mencit dilakukan terhadap kelompok perlakuan dan diketahui bahwa jumlah sel beta yang hidup pada kelompok air seduhan biji rambutan dosis tinggi hampir mendekati kelompok kontrol positif. Dapat disimpulkan bahwa khasiat air seduhan biji rambutan dosis 3,12 gram/kg bb tidak berbeda secara signifikan dengan glibenklamida 0,65 mg/kg bb.