Antimicrobial Activity and Gene Analysis of Plantaricin F of Lactobacillus Originated from Tropical Fruits
| Main Authors: | YULINERY, TITIN, NURHIDAYAT, NOVIK |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2013
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/209 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/209/147 |
Daftar Isi:
- Lactobacillus isolated from tropical fruits can be used as probiotic and potential to prevent diseases in the digestive tract due to bacteriocin content. One of the bacteriocin called plantaricin F, is produced by Lactobacillus plantarum. Plantarisin F has antimicrobial activity against Gram positive and Gram negative bacteria. The aim of the research was to test the antibacterial activities of several Lactobacillus species on 5 bacteria isolates, and analyzed the gene expression of plantaricin F. Seven isolates of ten selected isolates could inhibit the growth of Gram positive and Gram negative bacteria. Lactobacillus TB (NK) produced the largest inhibition area of 2.05 cm on E.coli, and twice stronger than chloramphenicol control. As confirmation, the plantaricin F gene was analyzed using RT-PCR. The analysis result showed that the size of plantaricin F gene was 70 bp. The highest relative expression of plantaricin F gene was shown by the Lactobacillus Mar A2 isolate of 10,676 Au, followed by Lactobacillus TB (NK) of 2.9207 Au, and the lowest was Lactobacillus TB (CK) of 0.5141 Au. Lactobacillus TB (NK) can be used as candidate of probiotic and alternative antibiotic in preventing of gastrointestinal disease.
- Lactobacillus yang berasal dari buah-buahan tropis dapat digunakan sebagai probiotik dan berpotensi mencegah penyakit pada saluran pencernaan karena menghasilkan bakteriosin. Salah satu bakteriosin adalah plantarisin F yang dihasilkan oleh Lactobacillus plantarum. Plantarisin F mempunyai aktivitas antimikroba terhadap bakteri Gram positif maupun Gram negatif. Penelitian ini bertujuan untuk menguji aktivitas antibakteri beberapa isolat Lactobacillus terhadap 5 bakteri uji dan menganalisa ekspresi gen plantarisin F. Dari sepuluh isolat Lactobacillus terpilih, tujuh isolat mampu menghambat bakteri Gram positif dan Gram negatif. Isolat TB (NK) memiliki daya hambat paling besar terhadap E. coli yakni 2,05 cm, dan dua kali lebih kuat dibandingkan kontrol kloramfenikol. Sebagai konfirmasi dilakukan analisis gen plantarisin F dengan RT-PCR. Hasil pengujian RT-PCR menunjukkan bahwa ukuran gen plantarisin F adalah ± 70 bp. Nilai ekspresi relatif gen plantarisin F paling tinggi ditunjukkan oleh isolat Mar A2 dengan nilai 10,676 Au, diikuti oleh isolat TB (NK) dengan nilai 2,9207 Au, sedangkan isolat TB (CK) memiliki nilai ekspresi relatif gen plantarisin F paling rendah yaitu sebesar 0,5141 Au. Isolat Lactobacillus TB (NK) dapat dijadikan kandidat probiotik dan antibiotik alternatif untuk mencegah penyakit saluran pencernaan