Correlation Analysis between Lymphocyte Proliferation Effect with Phenolic and Flavonoid Contents of Ethyl acetate Subfraction of Myrmecodia tuberosa (Non Jack) Bl. in BALB/C Mice, In Vitro
| Main Authors: | KHUMAIDI, AKHMAD, HERTIANI, TRIANA, SASMITO, EDIATI |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Indonesia
, 2017
|
| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/132 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/132/92 |
Daftar Isi:
- Ethyl acetate fraction of Myrmecodia tuberosa (non Jack) Bl. expressed potential immunomodulatory effect in preliminary study. This study was aimed to determine the stimulation index of each subfraction towards lymphocyte of BALB/C mice by in vitro technique and to explore the activityof lymphocyte proliferation correlated to total phenolic contents. lymphocyte proliferation assay was performed by MTT. Determination of total flavanoid content was done by a colorimetric method while the total phenolic was tested by the Folin-Ciocalteu method. The result showed stimulation index profile was positively correlated with the total flavanoid content( R2 = 0,606) while weak correlation was observed on the total phenolic content (R2 = 0,167).
- Fraksi etil asetat tumbuhan sarang semut (Myrmecodia tuberosa (non Jack) Bl.) memiliki efek sebagai imunomodulator berdasarkan penelitian sebelumnya. Penelitian ini bertujuan untuk menentukan indeks stimulasi dari masing-masing subfraksi terhadap aktivitas proliferasi sel limfosit mencit BALB/C secara in vitro dan untuk mengetahui aktivitas proliferasi sel limfosit tersebut berkorelasi dengan lemah (R2 = 0,167).