Effect of Nigella sativa Ethanol Extract on the Nitric Oxide Content and Renal Arteriole Diameter of a Pre-eclampsia Mouse Model
| Main Authors: | Purnamayanti, Ni Made Dwi, Windu, Siti Candra, Poeranto, Sri |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
The Atatürk University School of Medicine
, 2018
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| Subjects: | |
| Online Access: |
http://repository.poltekkes-denpasar.ac.id/3146/1/Effect%20of%20Nigella%20sativaEthanol%20Extract%20on%20the%20Nitric%20Oxide%20Content.pdf http://repository.poltekkes-denpasar.ac.id/3146/ https://www.eajm.org/sayilar/210/buyuk/148-151.pdf |
Daftar Isi:
- Objective: The aim of this study was to investigate the effect of Nigella sativa ethanol extract on nitric oxide (NO) levels and renal arteriole diameter of a pre-eclampsia mouse model. Materials and Methods: This experimental study was conducted using a post-test only control group design. Thirty BALB/c mice were divided into six groups: one negative control (normal pregnant mice), one positive control (pre-eclampsia model), and four groups of pre-eclampsia mice treated with varying doses of N. sativa (500 mg/kg body weight (BW)/day, 1000 mg/kg BW/day, 1500 mg/kg BW/day, and 2000 mg/kg BW/day). Ethanol extract of N. sativa was given for 5 days. Data were analyzed using analysis of variance. Results: We detected significant differences in NO levels between the pre-eclampsia mouse model and those given the ethanol extract. The latter had NO levels of 85.77±4.47 μM (500 mg/kg BW/day), 189.04±6.01 μM (1000 mg/kg BW/day), 226.56±2.13 μM (1500 mg/BW/day), and 207.98±4.74 μM (2000 mg/kg BW/day). The mean renal arteriole diameter showed significant differences among the treatment groups with N. sativa doses of 1000, 1500, and 2000 mg (15.15±2.21b μm, 16.35±2.52b μm, and 15.76±3.03b μm, respectively). Conclusion: N. sativa ethanol extract treatment increases NO levels and enlarges renal arteriole diameter of a pre-eclampsia mouse model in a dose-dependent manner. Keywords: Nitric oxide, Nigella sativa, pre-eclampsia, renal arteriole diameter