Post Thawing Sperm Quality and Ca+2 Intensity Characters of Local Goat Sperm After Freezing by Simple Method Using Deep Freezing
| Main Authors: | Ciptadi, Gatot, Ihsan, Muhammad Nur, Rahayu, Sri, Wahjuningsih, Sri, Munazaroh, Anis Mei, Putra, R. P. |
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| Other Authors: | Research Grant of HIKOM year 2015/2016) from Directorat General of High Education-DIKTI, Ministry of Education Republic of Indonesia. Contract No. 253.102/UN10.21/PG/2016, 18 February 2016 |
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya
, 2018
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| Subjects: | |
| Online Access: |
https://rjls.ub.ac.id/index.php/rjls/article/view/162 https://rjls.ub.ac.id/index.php/rjls/article/view/162/164 |
Daftar Isi:
- The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a post-thawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of -45°C then -196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca+2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca++ intensity was analysis descriptively. The result showed that post-thawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at -196°C. However freezing sperm conserved in -196°C is better than in -45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca+2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 μm each. It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.