Development of Spectrophotometric Methods for Iodide Determination Using Hydrogen Peroxide (H2O2) as Oxidizing
| Main Authors: | Lema, Abner Tonu, Sulistyarti, Hermin, Atikah, Atikah |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Natural B, Journal of Health and Environmental Sciences
, 2014
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| Subjects: | |
| Online Access: |
https://natural-b.ub.ac.id/index.php/natural-b/article/view/253 https://natural-b.ub.ac.id/index.php/natural-b/article/view/253/212 https://natural-b.ub.ac.id/index.php/natural-b/article/downloadSuppFile/253/103 |
Daftar Isi:
- Serious disorders of iodine deficiency can cause irreversible cretinism, pregnancy complications, goiter, compromised thyroid hormone production, mental impairment and decreasing cognitive function. In order to detect early indication of Iodine Deficiency Disorders (IDD) a development of simple spectrophotometric method for iodide determination is proposed using hydrogen peroxide (H2O2) as an oxidizing agent. Determination of iodide is based on the oxidation reaction of I- to I2 that form blue complex compound I2-starch. The blue color intensity of the complex I2-starch produced was measured at wavelength of 611 nm. Several parameters were optimized: complex formation and oxidation time of iodide, the optimum concentration of oxidizing agent, H2SO4, starch and effect of interfering ions (chloride, bromide, thiocyanate), with optimum conditions obtained of respectively: 10 minute, H2O2 0.1 M, H2SO4 0.05 M, starch 0.2 %. Under these condition, the method showed linearity of 5 - 60 ppm, limit of detection and quantification of 0.62 and 2.09 mg/L, respectively. This method has been successfully applied to synthetic and urine samples and can be recommended for the detection of iodide in patients hyperthyroidism and KI tablets.