Antibacterial Activity of Marine Microalgae Navicula salinicola Extract Against Propionibacterium acnes and Staphylococcus epidermidis
| Main Authors: | Kurnia, Dewi, Sari, Fitri Bella Mustika, Budiana, Wempi |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University
, 2020
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| Online Access: |
http://jkk.unjani.ac.id/index.php/jkk/article/view/65 http://jkk.unjani.ac.id/index.php/jkk/article/view/65/38 |
Daftar Isi:
- Navicula salinicola merupakan salah satu jenis mikroalga laut yang termasuk dalam kelas Bacillariophyceae (diatom). Mikroalga ini bersifat bentik dan memiliki warna agak kecoklatan. Pada penelitian ini dilakukan uji aktivitas antibakteri dari mikroalga Naviculla salinicolla yang diekstraksi menggunakan beberapa pelarut organik. Adanya aktivitas antibakteri dilihat melalui nilai KHM dan KBM dari masing-masing ekstrak serta mengidentifikasi golongan senyawa aktif yang terkandung dalam ekstrak tersebut. Ekstraksi dilakukan dengan metode maserasi bertingkat menggunakan pelarut n-heksana, kloroform, dan etanol. Uji aktivitas antibakteri dilakukan terhadap bakteri Propionibacterium acnes dan Staphylococcus epidermidis menggunakan metode mikrodilusi dengan klindamisin sebagai standar pembanding. Hasil penelitian menunjukkan ekstrak kloroform memiliki aktivitas antibakteri dengan nilai KHM 1.024 μg/mL dan nilai KBM >16.384 μg/ml. Pemantauan ekstrak kloroform menunjukkan adanya senyawa alkaloid, fenol, flavonoid, steroid dan terpenoid. Hasil pengujian bioautografi terhadap ekstrak kloroform Navicula salinicola menunjukan bahwa golongan senyawa fenol dan flavonoid memberikan aktivitas antibakteri. Kata kunci: aktivitas antibakteri, Naviculla salinicola, Propionibacterium acnes, Staphylococcus epidermidis
- Navicula salinicola is a type of marine microalgae that is included in the class Bacillariophyceae (diatoms). These microalgae are benthic and have a slightly brownish color. In this study, the antibacterial activity test of the Naviculla salinicolla microalgae was extracted using several organic solvents. The presence of antibacterial activity was seen through the MIC and MBC values of each extract and identified the active compound groups contained in the extract. Extraction was carried out by graded maceration method using n-hexane, chloroform, and ethanol as solvents. Antibacterial activity test was carried out against Propionibacterium acnes and Staphylococcus epidermidis using the microdilution method with clindamycin as the standard for comparison. The results showed that the chloroform extract had antibacterial activity with a MIC value of 1.024 μg / mL and a MBC value> 16.384 μg / ml. Monitoring of chloroform extract with TLC method showed the presence of alkaloids, phenols, flavonoids, steroids and terpenoids. The results of bioautographic testing on the chloroform extract of Navicula salinicola showed that the phenol and flavonoid compounds gave antibacterial activity Keywords: antibacterial activity, Naviculla salinicola, Propionibacterium acnes, Staphylococcus epidermidis