Antimicrobial Activity of Essential Oil from Centella asiatica (L.) Urb Plant
| Main Authors: | Jasmansyah, Jasmansyah, Fitriyani, Pipit, Sujono, Hernandi, Aisyah, Lilis Siti |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University
, 2020
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| Online Access: |
http://jkk.unjani.ac.id/index.php/jkk/article/view/54 http://jkk.unjani.ac.id/index.php/jkk/article/view/54/32 |
Daftar Isi:
- (Centella asiatica (L.) Urb) is one of the medicinal plants that has properties such as overcoming fever, anti-allergic, anti-inflammation, and central nervous system stimulants.The chemical compounds contained in the essential oil of C. asiatica are known to act as antibacterial. This study was to determine the antimicrobial activity of C.asiatica essential oil on the growth of Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans. The method used by the steam-water distillation method, the distillate results were carried out by Gas Chromatography - Mass Spectrometry and antimicrobial activity by microdilution method. The results showed that oil yield was 0,01% and with 37 compound components, 5 components of the main compounds with the highest concentration were (E)-β-farnesene (17,85%), Caryophyllene (13,55%), Germacrene-D (9,61%), α-Humulene (9,54%), β-Elemene (7,80%). The antimicrobial test results of C.asiatica essential oil on S.epidermidis bacteria with MIC and MBC ≤ 0,2%, P.aeruginosa bacteria with MIC and MBC values respectively 50% and 100%, and C.albicans fungi with MIC and MBC 100%. The results showed that essential oils of C. asiatica were more influential and had the potential to inhibit the growth of Gram positive bacteria compared to the inhibition of Gram negative bacteria and fungi. Keywords : Centella asiatica (L.) Urb , Essential Oils, Antimicrobials
- Pegagan (Centella asiatica (L.) Urb) merupakan salah satu tanaman obat yang memiliki khasiat seperti mengatasi demam, antialergi, anti inflamasi, dan stimulan sistem syaraf pusat. Kandungan senyawa kimia yang terdapat dalam minyak atsiri tanaman pegagan (C. asiatica) diketahui berperan sebagai antibakteri. Penelitian ini adalah untuk mengetahui aktivitas antimikroba dari minyak atsiri tanaman pegagan (C.asiatica) terhadap pertumbuhan bakteri Staphylococcus epidermidis, Pseudomonas aeruginosa, dan jamur Candida albicans. Metode yang digunakan metode destilasi uap-air, hasil destilat dilakukan uji Gas Chromatography – Mass Spectrometry dan aktivitas antimikroba dengan metode mikrodilusi. Hasil penelitian menunjukkan bahwa rendemen minyak sebesar 0,01% dan dengan 37 komponen senyawa, 5 komponen senyawa utama dengan konsentrasi tertinggi yaitu (E)-β-farnesen (17,85%), Kariofilen (13,55%), Germacrene-D (9,61%), α-Humulene (9,54%), β-Elemen (7,80%). Hasil uji aktivitas antimikroba minyak atsiri tanaman pegagan terhadap bakteri S.epidermidis dengan KHM dan KBM ≤ 0,2 %, bakteri P.aeruginosa dengan nilai KHM dan KBM berturut-turut sebesar 50 % dan 100% dan jamur C.albicans dengan nilai KHM dan KBM 100 %. Hasil menunjukkan bahwa minyak atsiri tanaman pegagan lebih berpengaruh dan berpotensi dalam menghambat pertumbuhan bakteri Gram positif dibandingkan dengan perhambatan terhadap bakteri Gram negatif dan jamur. Kata Kunci : Centella asiatica (L.) Urb, Minyak Atsiri, Antimikroba