Hydrolysis of Sorghum Seed Pulp with Microwave for the Production of Reducing Sugar as Raw Material of Bioethanol
| Main Authors: | Kolo, Sefrinus M. D., Edi, Eduardus |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Pertanian, Universitas Timor
, 2018
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| Online Access: |
https://savana-cendana.id/index.php/SLK/article/view/596 https://savana-cendana.id/index.php/SLK/article/view/596/267 |
Daftar Isi:
- Sorghum is a cereal plant that can grow in various environmental conditions so that it is potential to be developed, especially on dry climate marginal land in Indonesia. Sweet sorghum has great potential to be used as raw material for ethanol besides sugar cane. Cultivation of sweet sorghum has shifted from food and feed raw materials to industrial raw materials, both small and large scale. This study aims to obtain reducing sugar levels as raw material for bioethanol through hydrolysis using a microwave. As much as 10 grams of sorghum powder is suspended with dilute H2SO4 solution of 0.1 N, 0.3 N and 0.5 N as much as 250 mL and then heated using microwave irradiation at a temperature of 200 oC for 20 and 40 minutes. The liquid fraction produced by hydrolysis was analyzed by reducing sugar content with the DNS method using a UV-Vis spectrophotometer. The results obtained showed that the optimum hydrolysis conditions using microwave were achieved at concentrations of H2SO4 0.5 N at 200 oC and hydrolysis time at 40 minutes. Reducing sugar content of 42.71 mg / L. The sugar content rises by 19.95% compared to the hydrolysis time of 20 minutes.
- Sorgum merupakan tanaman serealia yang dapat tumbuh pada berbagai keadaan lingkungan sehingga potensial dikembangkan, khususnya pada lahan marginal beriklim kering di Indonesia. Sorgum manis mempunyai potensi yang besar digunakan sebagai bahan baku etanol selain tebu. Budidaya sorgum manis mengalami pergeseran dari bahan baku pangan dan pakan menjadi bahan baku industri, baik skala kecil maupun skala besar. Penelitian ini bertujuan untuk mendapatkan kadar gula pereduksi sebagai bahan baku bioethanol melalui hidrolisis menggunakan microwave. Sebanyak 10 gram serbuk sorgum disuspensi dengan larutan H2SO4 encer 0,1 N, 0,3 N dan 0,5 N sebanyak 250 mL lalu dipanaskan menggunakan iradiasi microwave pada temperatur 200 oC selama 20 dan 40 menit. Fraksi cair hasil hidrolisis dianalisis kandungan gula pereduksi dengan metode DNS menggunakan spektrofotometer UV-Vis. Hasil yang diperoleh menunjukkan bahwa kondisi optimum hidrolisis menggunakan microwave dicapai pada konsentrasi H2SO4 0,5 N temperatur 200 oC dan waktu hidrolisis 40 menit. Kadar gula pereduksi sebesar 42,71 mg/L. Kadar gula naik sebesar 19,95% dibanding waktu hidrolisis 20 menit.