Effect of hypoxia-inducible factor-1α induction by CoCl2 on breast cancer cells survival: influence of cytochrome-c and survivin
| Main Authors: | Paramita, Reni; Doctoral student in Biomedical Study, Faculty of Medicine, Universitas Indonesia, Jakarta, Sadikin, Mohamad; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Sutandyo, Noorwati; Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Jakarta, Wanandi, Septelia I.; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Medicine Universitas Indonesia
, 2014
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| Subjects: | |
| Online Access: |
http://mji.ui.ac.id/journal/index.php/mji/article/view/933 http://mji.ui.ac.id/journal/index.php/mji/article/view/933/985 |
Daftar Isi:
- Background: Tumor tissue usually became hypoxic due to disruption of oxygen supply. Adaptation response to hypoxia is mediated by transcription factor, hypoxia- inducible factor-1α (HIF-1α). HIF-1α signaling is known to increase the expression of pro-apoptotic protein cytochrome-c, and anti- apoptotic survivin. In this study we wanted to analyze the role of HIF-1α on breast cancer cells survival through pro-apoptosis cytohrome-c and anti-apoptosis survivin regulation.Methods: Breast cancer cell lines T47D were induced by CoCl2 then harvested to analyze the expression of HIF-1α, protein cytochrome-c, mRNA survivin and cell viabilities.Results: HIF-1α induction by CoCl2 causes the increase of protein and mRNA of HIF-1α, cytochrome-c protein, and survivin mRNA, but does not cause the changes in cell viability.Conclusion: HIF-1α induction have no effects on breast cancer cell line T47D viabilities due to the balance regulation between pro-apoptosis expression cytochrome-c and anti-apoptosis survivin.