IDENTIFICATION OF DNA MARKERS LINKED TO CMV RESISTANCE GENE(S)IN HOT PAPPER
| Main Authors: | Rustikawati , Rustikawati, Herison, Catur, Sudarsono , Sudarsono, Eliyanti, Eliyanti, Suryati, Dotti |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Program Studi Agronomi Fakultas Pertanian Universitas Bengkulu
, 2014
|
| Subjects: | |
| Online Access: |
http://repository.unib.ac.id/7350/1/Akta%20Agrosia%2011%282%29%202008.pdf http://repository.unib.ac.id/7350/ |
| ctrlnum |
7350 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://repository.unib.ac.id/7350/</relation><title>IDENTIFICATION OF DNA MARKERS LINKED TO CMV RESISTANCE GENE(S)IN HOT PAPPER</title><creator>Rustikawati , Rustikawati</creator><creator>Herison, Catur</creator><creator>Sudarsono , Sudarsono</creator><creator>Eliyanti, Eliyanti</creator><creator>Suryati, Dotti</creator><subject>S Agriculture (General)</subject><description>cMV has caused severe damages in fl?Hf""J ,,,, intbction ,:aused major yieid reduction in Indonesia, Inheritance study on resistance to CMV showcd that resistance 1o CMV was co(rtrolled by at least three recessiYe genes. The objective of this study was to idenrifu i)NA rnarkers linked to CMV resistance gene(s) in hot pepper using bulk segregant analysis (BSA) sh'ategy. Molecular markers were cleveloped by RAPD analysis on the F2 generation generated from a cross between C 102,i and, C frutesscetil as rhe mapping population' DNAgenomeswereisolatedfromseventytworandornlysampledpiantsofasegregatedF2popriition and were amplified with six groups of random primers from Operon Technologies of OpA, OpC, OpE, Opt OpFl and OPM each ofwhich consisted of 20 primers. Result of the experiment obtained 20 CMV resistance specific
RAPD markers. These RAPD markers are grouped ^nto two linked groups and one of the nrarkers (OpH5ro) r,r.as
shown to be associated with one of the three CMV resistar. ce gerres with the log-lil.:elihood (LOD) vatue of:.6+.
The OPH5,* marker linked to a CMV resistance gene with a distance of 8.1 cM, and may be used to assist hot
pepper breeding programs lbr CMV resistance.</description><publisher>Program Studi Agronomi Fakultas Pertanian Universitas Bengkulu</publisher><date>2014</date><type>Journal:Article</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><rights>cc_gnu_gpl</rights><identifier>http://repository.unib.ac.id/7350/1/Akta%20Agrosia%2011%282%29%202008.pdf</identifier><identifier> Rustikawati , Rustikawati and Herison, Catur and Sudarsono , Sudarsono and Eliyanti, Eliyanti and Suryati, Dotti (2014) IDENTIFICATION OF DNA MARKERS LINKED TO CMV RESISTANCE GENE(S)IN HOT PAPPER. Akta Agrosia, 11 (2). pp. 108-112. ISSN 1410-3354 </identifier><recordID>7350</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal PeerReview:PeerReviewed PeerReview Book:Book Book |
| author |
Rustikawati , Rustikawati Herison, Catur Sudarsono , Sudarsono Eliyanti, Eliyanti Suryati, Dotti |
| title |
IDENTIFICATION OF DNA MARKERS LINKED TO CMV RESISTANCE GENE(S)IN HOT PAPPER |
| publisher |
Program Studi Agronomi Fakultas Pertanian Universitas Bengkulu |
| publishDate |
2014 |
| topic |
S Agriculture (General) |
| url |
http://repository.unib.ac.id/7350/1/Akta%20Agrosia%2011%282%29%202008.pdf http://repository.unib.ac.id/7350/ |
| contents |
cMV has caused severe damages in fl?Hf""J ,,,, intbction ,:aused major yieid reduction in Indonesia, Inheritance study on resistance to CMV showcd that resistance 1o CMV was co(rtrolled by at least three recessiYe genes. The objective of this study was to idenrifu i)NA rnarkers linked to CMV resistance gene(s) in hot pepper using bulk segregant analysis (BSA) sh'ategy. Molecular markers were cleveloped by RAPD analysis on the F2 generation generated from a cross between C 102,i and, C frutesscetil as rhe mapping population' DNAgenomeswereisolatedfromseventytworandornlysampledpiantsofasegregatedF2popriition and were amplified with six groups of random primers from Operon Technologies of OpA, OpC, OpE, Opt OpFl and OPM each ofwhich consisted of 20 primers. Result of the experiment obtained 20 CMV resistance specific
RAPD markers. These RAPD markers are grouped ^nto two linked groups and one of the nrarkers (OpH5ro) r,r.as
shown to be associated with one of the three CMV resistar. ce gerres with the log-lil.:elihood (LOD) vatue of:.6+.
The OPH5,* marker linked to a CMV resistance gene with a distance of 8.1 cM, and may be used to assist hot
pepper breeding programs lbr CMV resistance. |
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