SELEKSI PRIMER RAPD UNTUK KETAHANAN TERHADAP CMV: PENGGUNAAN STRATEGI BSA (BULK SEGREGANT ANALYSIS)
| Main Authors: | Rustikawati , Rustikawati, Herison, Catur, Eliyanti, Eliyanti, Sudarsono , Sudarsono |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Program Studi Agronomi Fakultas Pertanian Universitas Bengkulu
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unib.ac.id/7333/1/Akta%20Agrosia%2010%282%29%202007.pdf http://repository.unib.ac.id/7333/ |
Daftar Isi:
- RApD primers selection have to be done in order to identi$ RAPD markels]inkage with CMV resistance on hot pepper (Capsicum sp). The objective of this research was to identifr RAPD markers polymorphic with CMV resisiance through bulk segregant analysis (BSA) approach. Random primers used in this research were 120 primers from six op.ron group., tt "y *.r. oPA, OPC, OPE, OPR OPH an{ OPM- The mapping population was generated from a cross if a'Capstcum annuum resistant genotype (C1024) x a susceptible C frutescens g.noiyp.. The DNA of the most r"lirtunt and susceptible F2 plants were pooled respectively and amplified *itf, tG random primers. The result showed that out of 120 random primers, 25 primers showed polymorphism on resistant versus susceptible pool DNA. They were OPA-7, OPA-13, OPA-15, OPA-20, OPC-4, OPC-s' opc-6, opc-8, opE-l, opE-6;oPE-7,oPE-l2,oPF-14, oPF-6, oPH-3, oPH-s, oPH-10, oPH-12, oPH- 13, OPH-I8, OPM-7, OPM-I8, and OPM-20