Plant Regeneration from Leaf Blade Explants of Horseradish (Amoracia rusticana L.) through In Vitro Culture
| Main Author: | Yuliadi, E. |
|---|---|
| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Program Studi Agronomi Fakultas Pertanian Universitas Bengkulu
, 2008
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| Subjects: | |
| Online Access: |
http://repository.unib.ac.id/269/1/Erwin.pdf http://repository.unib.ac.id/269/ http://faperta.unib.ac.id |
Daftar Isi:
- Horseradish as an important crop in Japan is difficult to be propagated by using seed. The objective of this research was to overcome such difficulty through in vitro culture. The explants used were young leaves and old leaves. The MS basal medium was used and treated with 0; 0.1; 0.3; 0.6; 1.0 and 10 μM 2,4-D combinated with 0; 0.1; and 1.0 μM BA. This experiment showed that a combination of 0.1 μM 2,4-D and 0,.1 μM BA gave the highest plantlet regeneration as compared with 0; 1.0 and 10 μM 2,4-D combinated with 0 and 1.0 μM BA. Plantlet regeneration took place through direct morphogenesis. Sub-culturing calli from 1.0 μM 2,4-D and 0; 0.1 and 1.0 μM BA-supplemented medium to a medium without 2,4-D nor BA was the best sub-culturing method to get optimal plantlet regeneration. Young leaf explants had better response to plant growth regulators compared with old leaf explants.