Optimasi Pertumbuhan dan Multiplikasi Lini Klon PLBs Anggrek Spathoglottis plicata Blume melalui Modifikasi Komposisi Medium MS dan Sitokinin
| Main Authors: | Romeida, Atra, Surjono, H. Sutjahjo, Agus, Purwito, Dewi, Sukma, Rustikawati, Rustikawati |
|---|---|
| Format: | Article PeerReviewed Archive |
| Bahasa: | eng |
| Terbitan: |
, 2013
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| Subjects: | |
| Online Access: |
http://repository.unib.ac.id/11722/1/B4%20ATRA%20JHI%20JURNAL.pdf http://repository.unib.ac.id/11722/ |
Daftar Isi:
- In order to enhance the multiplication of Protocorm Like Bodies (PLBs) of Spathoglottis plicata orchid in vitro, several combinations of vitamin, sucrose concentration, and cytokinin were tested. This experiment was arranged in Completely Randomized Design (CRD) with two factors. The first factor was combination of vitamin composition and sugar concentrations in the medium which consisted of four different combinations (J1 = vitamin MS + sucrose 30 g L -1 -1 , J2 = vitamin B5 + sucrose 30 g L , J3= vitamin MS + sucrose 40 g L -1 -1 , J4 = vitamin B5 + sucrose 40 g L ). The second factor was seven combinations of cytokinin types and its concentrations (S0 = without cytokinin (control), S1 = 20 μ M BA, S2 = 40 μ M BA, S3 = 20 μ M kinetin, S4 = 40 μ M kinetin, S5 = 75 ml L -1 coconut milk, and S6 = 150 ml L -1 coconut milk). PLBs were used as explant and were grown on MS solid medium containing various vitamin, sucrose, and cytokinin combinations as mentioned above. Growth and multiplication of PLBs were based on the number of PLBs per explant, number of plantlets per explant, number of roots formed, plantlet height and visual appearance and performance of the observations at 6 MST. The best growth and multiplication of PLBs orchid S. plicata was produced on MS medium modified with B5 vitamins and 30 g L-1 -1 sucrose, followed by those and on MS medium enriched with 75 ml L coconut milk and on MS medium supported by 20 μ M BA.