Variasi Genetik Mutan Anggrek Spathoglottis plicata Blume. Berdasarkan Marker ISSR
| Main Authors: | Romeida, Atra, Surjono, H. Sutjahjo, Agus, Purwito, Dewi, Sukma, Rustikawati , Rustikawati |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
PERAGI dan Departemen Agronomi dan Hortikultura Fakultas Pertanian IPB
, 2012
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| Subjects: | |
| Online Access: |
http://repository.unib.ac.id/1130/1/6829-19209-1-PB.pdf http://repository.unib.ac.id/1130/ |
Daftar Isi:
- Mutants of Spathoglottis plicata Blume were obtained from plants treated with Gamma Irradiation at 30-100 Gray. The mutants showed variations in the flower morphology. The objective of this experiment was to identify genetic variations of orchids S. plicata and its mutants using inter-simple sequent repeat (ISSR) markers. The amplified product of 10 ISSR primers produced 360 bands and 71 ISSR of the loci (90.14%) were polymorphic. The coefficient of similarity and principal component analysis produced five major groups with similarity coefficient of 0.68. The goodness of fit correlation matrix value reached 0.91. Therefore the ISSR isa good marker for identification of S. plicata mutants.