The Potential of Pseudomonas sp. in Controlling Bacterial Leaf Blight Disease (Xanthomonas oryzae pv. oryzae) by In Vitro
| Main Authors: | Saylendra, Andree, Nurmayulis, Nurmayulis, Ahdiani, Pina |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Universitas Bangka Belitung
, 2017
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| Online Access: |
https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/5 https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/5/5 |
Daftar Isi:
- This research was aimed to determine the potential of Pseudomonas sp. in controlling bacterial leaf blight disease (Xanthomonas oryzae pv. oryzae) in vitro. This research was conducted at the Laboratory of Agroecology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa on June to September 2014. This research implemented experimental one factor that arranged in Completely Randomized Design with five replications. The treatment consisted of 6 isolates of Pseudomonas sp. (Ps 6, Ps 29, Ps 39, Ps 40, Ps 45, dan Ps 46) which was screened from the rhizosphere of roots of paddy plants. The inhibition of Pseudomonas sp. bacteria isolates was tested and showed that the results were not significantly different neither it was between each isolate nor against control (without treatment bacteria). Ps 39 isolate tend to had higher clear zone compare to other isolates.
- Tujuan penelitian ini untuk mengetahui potensi Pseudomonas sp. asal rhizosper padi sebagai agen hayati untuk mengendalikan penyakit hawar daun bakteri (Xanthomonas oryzae pv. oryzae) secara in vitro. Penelitian dilaksanakan di Laboratorium Agroekoteknologi, Fakultas Pertanian, Universitas Sultan Ageng Tirtayasa pada bulan Juni-September 2014. Penelitian menggunakan rancangan acak lengkap non faktorial dengan lima ulangan. Perlakuan terdiri dari 6 isolat Pseudomonas sp. (Ps 6, Ps 29, Ps 39, Ps 40, Ps 45, dan Ps 46) yang memiliki kemampuan antagonis dan 1 kontrol air (tanpa bakteri) sebagai pembanding, sehingga terdapat 35 satuan percobaan. Isolat-isolat bakteri Pseudomonas sp. yang diuji daya hambatnya secara analisis tidak memiliki pengaruh, baik antar isolat maupun terhadap kontrol (tanpa bakteri perlakuan). Namun, secara keseluruhan mempengaruhi yaitu dengan terbentuknya zona bening menunjukkan adanya tingkat pengendalian. Isolat-isolat bakteri Pseudomonas sp. memiliki kemampuan yang relatif sama dalam mengendalikan bakteri Xanthomonas oryzae pv. oryzae. Meski demikian, isolat Ps 39 dan memiliki zona bening yang cenderung lebih tinggi.