Potency of Bamboo Root Endophytes as Biocontrol to Fusarium oxysporum Pathogen Cause Yellowing Disease on Pepper Plant
| Main Author: | Ropalia, Ropalia |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Universitas Bangka Belitung
, 2017
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| Online Access: |
https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/11 https://agrosainstek.ubb.ac.id/index.php/agrosainstek/article/view/11/11 |
Daftar Isi:
- The yellowing disease on pepper plant (Pipper nigrum L) caused by plant-parasitic nematodes and F. oxysporum infection is a major disease in Bangka island. The wound that caused by plant-parasitic nematodes will facilitate infection into roots by the pathogen of F. oxysporum easily. This caused the plant sensitive to drought and nutrient deficiency. Utilization of endophytic microbes is one of biological control that environmental friendly and to support sustainable agriculture. The aim of this study was to explore endophytic isolates can inhibit the growth of F. oxysporum in vitro. The endophytes were isolated from bamboo root and selected their antagonistic potential against F.oxysforum by dual culture methods. The study resulted an endophytic bacteria and 13 isolates of endophytic fungi that inhibit mycelium growth of F. oxysforum. The antagonistic activity of endophytic bacteria to F. oxysforum is 28.25% by antibiosis mechanism and endophytic fungi, about 11.00-58.25% by space colonization and nutrition competition on substrate.
- Penyakit kuning pada lada (Pipper nigrum L) yang disebabkan oleh infeksi nematoda parasit tanaman dan F. oxysporum spp. merupakan penyakit utama di Bangka. Luka akibat serangan nematoda parasit akan memudahkan infeksi F. oxysporum spp. Hal Ini menyebabkan tanaman peka terhadap kekeringan dan kekurangan unsur hara. Pemanfaatan mikrob endofit merupakan salah satu pengendalian hayati ramah lingkungan dan mendukung pertanian berkelanjutan. Penelitian ini bertujuan mendapatkan isolat-isolat endofit yang mampu menghambat pertumbuhan patogen F. oxysporum secara in vitro. Endofit diisolasi dari akar akar bambu dan diuji potensi antagonisnya terhadap F.oxysforum dengan metode dual culture. Hasil diperoleh 1 isolat bakteri endofit dan 13 isolat cendawan endofit yang berpotensi menghambat pertumbuhan F. oxysporum secara in vitro. Kemampuan antagonisme bakteri endofit asal akar terhadap patogen F. oxysforum sebesar 28,25% dengan cara antibiosis dan cendawan endofit berkisar 11.00-58,25% melalui mekanisme kompetisi ruang dan nutrisi.