Animal Modeling Try Strain Balb/c Mice with Gardnerella Vaginalis
| Main Authors: | Sirait, Lenny Irmawaty, Kamelia, Emma, Simanjuntak, Tigor Peniel, Massi, Muh Nasrum, Rauf, Syahrul, Natzir, Rosdiana, Hatta, Mochammad, Rahayu, Sri, Aritonang, Tetty Rina |
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| Format: | Article PeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
Global Society of Scientific Research and Researchers
, 2017
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| Subjects: | |
| Online Access: |
http://repository.uki.ac.id/757/1/Animal%20Modeling%20Try%20Strain%20Balbc%20Mice%20with%20Gardnerella%20Vaginalis.pdf http://repository.uki.ac.id/757/ http://gssrr.org/index.php?journal=JournalOfBasicAndApplied&page=index |
Daftar Isi:
- Gardnerella vaginalis is the causative agent of sexually transmitted infections (STIs) of the bacterial class and is the main species of bacterial vaginosis (BV), classified as gram-negative bacteria, and is anaerobic facultative. The incidence of bacterial vaginosis increased rapidly in Indonesia with a prevalence of approximately 20-30% of women of childbearing age suffering from sexually transmitted infections diagnosed BV and an increase of 50-60%. BV is the leading cause of vaginal infection in women of childbearing age and is one of the most common infectious diseases in the gynecological obstetrics. Balb-C is a trying animal with a human-like cervical vagina. The Objective of this study is to identify the dose of Gardnerella vaginalis bacteria that has the potential to live on the vagina of experimental animals. Balb/c female strain, adult age 8-12 weeks, weight 20-25 g. Using the patient's urine specimen suspect Bacterial vaginosis was inoculated on intravaginal Balb-c each of 10 μl doses of 3 x 106, 3 x 105, and 3 x 104. Prior to inoculation (H0), a vaginal swab was taken and continued at H1 ± 18-24 h post-infection. After inoculation, 3x checks per day to control the health status include movement, respiration, food, fur state (neat or messy), for a period of 5 days and weight measured 1 day before and 5 days after infection. Vaginal ecosystem changes 18-24 hours post inoculation of intravaginal Gardnerella vaginalis indicated by growth of cultured culture colony on Plat count agar (PCA) ie 21 cfu / ml for dose 3 x 104; 106 cfu / ml for a dose of 3 x 105; And too much to count (± 10000) for a dose of 3 x 106. In conclusions; the dose `Concentration 3 x 104 can be recommended as a concentration of treatment of infection of experimental animals using Gardnerella vaginalis in subsequent studies