A Polymorphism of Insulin-Like Growth Factor Binding Protein 2 Gene Associated with Growth and Body Composition Traits in Kampong Chickens
| Main Authors: | Furqon, Ahmad, Gunawan, Asep, Ulupi, Niken, Suryati, Tuti, Sumantri, Cece |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
, 2018
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| Online Access: |
https://ojs.unud.ac.id/index.php/jvet/article/view/32901 https://ojs.unud.ac.id/index.php/jvet/article/view/32901/25095 |
Daftar Isi:
- Insuline-like growth factor binding protein 2 (IGFBP2) is one of the principal binding proteins that has biological functions involved in growth, development, and differentiation. Selection for rapid growth based on molecular Marker Assisted Selection is required to increase production performance. The present study was designed to analyze the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits. Kampong chicken, a native chicken in Indonesia, is slow-growing chicken. A total of 59 males were used in the current study. Growth and body composition were measured in 24 wk of age. Primers for intron 2 region were designed from genomic chicken sequence. A c.1032C>T SNP of the IGFBP2 gene intron 2 region was detected and PCR-RFLP method was then used to genotype Kampong chicken population. The result showed that IGFBP2 polymorphism was significantly associated with body, carcass, breast, breast muscle, pectoralis minor, leg, and wings weight in Kampong chicken population (P < 0.05). The research suggests that the IGFBP2 gene could be a candidate gene that affects growth and body composition traits in chicken.
- Insuline-like growth factor binding protein 2 (IGFBP2) merupakan protein pengikat utama yang memiliki fungsi biologi terlibat dalam pertumbuhan, perkembangan, dan diferensiasi. Program seleksi untuk sifat pertumbuhan cepat berdasarkan penanda molekuler sangat diperlukan untuk meningkatkan performa produksi. Terkait hal tersebut, penelitian ini didesain untuk menganalisis asosiasi keragaman gen IGFBP2 dengan sifat pertumbuhan dan komposisi tubuh pada ayam kampong. Ayam kampung merupakan ayam asli Indonesia dan tergolong ayam dengan pertumbuhan lambat. Sebanyak 59 ayam kampung jantan digunakan dalam penelitian ini. Pertumbuhan dan komposisi tubuh diukur pada umur 24 minggu. Sepasang primer didesain pada daerah intron 2 berdasarkan sekuen genom ayam. Sebuah SNP c.1032C>T pada gen IGFBP2 intron 2 dideteksi dan metode PCR-RFLP digunakan dalam menentukan genotype ayam kampung. Hasil menunjukkan bahwa keragaman IGFBP2 signifikan berasosiasi dengan bobot badan, karkas, dada, otot dada, pectoralis minor, paha, dan sayap pada populasi ayam kampung (P<0.05). Penelitian ini menyarankan gen IGFBP2 dapat dijadikan kandidat gen yang mempengaruhi sifat pertumbuhan dan komposisi tubuh pada ayam. Kata kunci: ayam kampung, pertumbuhan, komposisi tubuh, insuline-like growth factor binding protein 2, keragaman