Filogenetik Ular Sanca Batik (Malayopython reticulatus) Lokal Jawa Berdasar Sekuen Gen Sitokrom B (PHYLOGENETIC STUDY OF RETICULATED PYTHON (MALAYOPYTHON RETICULATUS) JAVAN LOCALITY BASED ON GENE CYTOCHROME B SEQUENCES)
| Main Authors: | Raharjo, Slamet, Hartati, Sri, Indarjulianto, Soedarmanto, Widayanti, Rini, Nururrozi, Alfarisa |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association
, 2018
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| Subjects: | |
| Online Access: |
https://ojs.unud.ac.id/index.php/jvet/article/view/29554 https://ojs.unud.ac.id/index.php/jvet/article/view/29554/25536 |
Daftar Isi:
- Reticulated python (Malayopython reticulatus) Javan locality has different phenotype among West Java, Central Java and East Java locality. This research aim was to study the phylogenetic of reticulated python (M. reticulatus) Java locality based on the partial gene cytochrome b sequences. Fifteen males and females adult healthy reticulated pythons (5 from West Java, 5 from Central Java and 5 from East Java) were used in this study. As much as 1 mL blood samples collected from ventral coccygeal vein, put in Eppendorf with EDTA then sent to Laboratory of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University for total Deoxyrybo Nucleic Acid (DNA) extraction and DNA amplification with Polymerase Chain Reaction (PCR) method. DNA sequencing was done in First Base Company Singapore by PT. Genetika Science Jakarta. The gene cytochrome b sequences were phylogenetically analysed using Neighbor joining (MEGA v. 5.0) method. The length of partial gene cytochrome b nucleotides sequence of M. reticulatus from West Java, Central Java and East Java locality was 819 bp. The phylogram using Neighbor joining method showed that Malayopython reticulatus Central Java locality was differentiated from West Java locality, while the East Java locality was undifferentiated from West Java and Central Java locality, namely in the same group. It could be concluded that reticulated python (M. reticulatus) Java locality has partial gene cytochrome b sequences with 819 bp. The phylogenetic of Malayopython reticulatus West Java and several from East Java were located at same branch with M. reticulatus GenBank, while the Central Java and several from East Java were located at different branch. It needs further research with more samples and validity of localities for better result.
- Ular sanca batik (Malayopython reticulatus) lokalitas Jawa memiliki fenotip yang berbeda antara lokalitas Jawa Barat, Jawa Tengah dan Jawa Timur. Penelitian ini bertujuan untuk mengetahui filogenetik ular sanca batik (Malayopython reticulatus) lokalitas Jawa berdasar sekuen gen sitokrom B. Sebanyak 15 ekor ular sanca batik dewasa sehat, jenis kelamin jantan dan betina (5 dari Jawa Barat, 5 dari Jawa Tengah dan 5 dari Jawa Timur) digunakan dalam penelitian ini. Sampel darah sebanyak 1 mL dikoleksi dari masing-masing ular dan ditampung dalam tabung eppendorf ber-EDTA, kemudian dikirim ke Laboratorium Biokimia FKH UGM untuk dilakukan isolasi total Deoxyrybo Nucleic Acid (DNA), desain primer, amplifikasi DNA dengan Polymerase Chain Reaction (PCR) dan Elektroforesis Gel Agarose. Sequencing DNA dilakukan di PT Genetika Science Jakarta. Data hasil sekuensing gen sitokrom B dianalisis untuk penentuan filogenetik dengan metode Neighbor joining (MEGA v. 5.0). Hasil sekuensing gen sitokrom B, Malayopython reticulatus lokalitas Jawa Barat, Jawa Tengah dan Jawa Timur memiliki keseragaman panjang nukleotida 819 bp. Hasil filogram menggunakan metode Neighbor joining menunjukkan Malayopython reticulatus lokalitas Jawa Tengah dapat dibedakan dari lokalitas Jawa Barat, sedang lokalitas Jawa Timur ada yang mirip lokalitas Jawa Tengah dan ada yang mirip lokalitas Jawa Barat. Malayopython reticulatus lokalitas Jawa Barat dan beberapa lokalitas Jawa Timur filogenetiknya lebih dekat dengan Malayopython reticulatus Gene Bank dibanding lokalitas Jawa Tengah. Dapat disimpulkan bahwa Malayopython reticulatus lokalitas Jawa memiliki panjang nukleotida 819 bp. Malayopython reticulatus lokalitas Jawa Barat dan sebagian lokalitas Jawa Timur filogenetiknya lebih dekat dengan Malayopython reticulatus Gene Bank. Ada perbedaan filogenetik Malayopython reticulatus lokalitas Jawa Tengah dengan lokalitas Jawa Barat dan Jawa Timur. Perlu penelitian lebih lanjut dengan jumlah sampel yang lebih banyak dan variasi asal-usul lokalitas.