Biochemical Characteristics of Chitinase Enzyme from Bacillus sp. of Kamojang Craater, Indonesia
| Main Author: | Natsir, Hasnah |
|---|---|
| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
Journal of Biochem. Molecular Biology and Biophys
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/9868 |
Daftar Isi:
- Abstract Chitinase and chitin deacetylase and enzyme capable of degrading chitin into chitooligomers and chitosan. The chitinase characterized and purified in this study were extracted from the acidophillic Bacillus sp isolated from Kamojang Creater West Java Indonesia. When grown in liquid media containing colloidal chitin, the optimum chitinase activity of the acidophillic isolate was reached after 4-5 days of incubation. The optimum temperatur and pH of the chitinase and chitin deacetylase were found at 37oC and pH 5. When incubated at pH 5, the activity of chitin deacetylase was increased; after 3 h, the activity was 1.5 times of the control. The enzyme was stable at pH 4, after 2 h incubation, the activity was still 80% of the control. The chitinase and chitin deachetylase activity, while chitin deacetylase activities were not influenced by Mg++ nor Ca++, Ni++ and Cu++ inhibited the chitinase activity, while chitin decetylase activity was not affected by Cu++ addition. When 1 mM of EDTA was added, the enzyme activity was reduced from 40 to 50%. Keywords: Chitinase, chitin deasetylase, Enzyme charanteristics, Incubation