Potential use of cholinesterase activity from tropical green mussel, Perna viridis as a biomarker in effect-based marine monitoring in Indonesia
| Main Author: | YAQIN, Khusnul |
|---|---|
| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
, 2011
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/829 |
Daftar Isi:
- Green mussels (Perna viridis) play an important economic and ecological role in the coastal areas of Indonesia. P. viridis has been used as an eco-sentinel organism for marine biomonitoring program in Asia regions. To magnify its competency as a relevant organism in biomonitoring, the cholinesterases of P. viridis from a selected coastal area of Indonesia have been characterized. Dissected organs which were gill, foot, mantle and posterior adductor muscle were examined for substrate specificity and inhibitors sensitivity using selective and non-selective substrates and inhibitors. The highest level of cholinesterase activity was observed in gill and followed by foot, mantle and posterior adductor muscle. The substrate specificity approach using various alkylthiocholines indicated that the cholinesterases of P. viridis constitute an acetylcholinesterase (AChE) and a butyrylcholinesterase (BuChE). Likewise, the inhibitors sensitivity approach using eserine, BW284C51, and iso-OMPA sustained the previous approach which recorded typical AChE and atypical BuChE. The application of cholinesterase activity in three selected areas of Indonesian waters suggested that the gill of P. viridis is the most suitable organ for employing the ChE activity as a biomarker in effect-based marine monitoring.