Beta Amyloid Peptide Role in Animal Modeling Trial of Alzheimer's Disease
| Main Author: | Emma Kamelia, Hadiyat Miko Marni Br Karo, Sinar Pertiwi, Cahyono Kaelan, Andi Asadul Islam, Mochammad Hatta |
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| Format: | Article |
| Terbitan: |
International Journal of Sciences: Basic and Applied Research
, 2017
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/24098 |
Daftar Isi:
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- The Objective of this paper is to investigate the profile changes of Amyloid Beta (A??) levels in the blood serum using AmyloidASerum (SAA)Mouse ELISA Kit and histopathological image of hippocampal tissue in mice injected with amyloid beta-protein (A??) fragment 1-42.Wistar rats were randomly selected and were divided into two cages. First Cage contained three mice which were injected A??1-42 peptide in the intracerebroventricullar (ICV) area with each dose of 0.2 ??g, 0.13 ??g and 0.1 ??g. While the second cage contained three mice which were injected A??1-42 peptide in intraperitoneal (IP) area with each dose of 0.2 ??g, 0.13 ??g and 0.1 ??g. Observation was done in a month. SAA mouse ELISA Kit Test and histopathologic image were used to detect A??1-42 peptide levels and the accumulation of amyloid plaques layer formation on the extracellular (senile) and neurofibrillary tangles (NFT) in the intracellular. profile changes showed that the injection of 0.2 ??g peptide A??1-42 in the intracerebroventricullar(ICV)area increased A?? levels compared to other doses both in the ICV and IP areas. PA results showed hypoxia state on nerve cells followed by vacuoles shrinkage in a large number of cells at 0.2 ??g injection dosage in the ICV area. The results presented showed the importance of the establishment of A??1-42 peptide injection doses in experimental animals to build a more complete understanding of the molecular mechanisms result of A??1-42 peptide exposure in injected mice in which the sign shown resemble Alzheimer's disease sign. Thus, various research efforts trials of the drug as well as natural ingredients intended for AD treatment can be growing