DETEKSI GEN VITAMIN D RECEPTOR (VDR) YANG DIEKSTRAKSI DARI SALIVA DENGAN METODE POLYMERASE CHAIN REACTION (PCR)
| Main Author: | Alvionita, Finny |
|---|---|
| Format: | Lainnya |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/11466 |
Daftar Isi:
- This research has conducted about " Detection of Vitamin D Receptor Gene ( VDR ) Extracted From Saliva With Polymerase Chain Reaction ( PCR ) Methods " . This research was conducted in the Laboratory of Molecular Biology and Immunology Section of Microbiology, Faculty of Medicine, Hasanuddin University. This study aims to determine the presence of the VDR gene in saliva by using PCR (Polymerase Chain Reaction). Salivary samples were extracted by the method of Oragene DNA so as to obtain genomic DNA. Furthermore, the genomic DNA was amplified with specific primers (F-5'-CTG GGG GGG AGC TAT GAG GAA GGA-3 ', R-5'-GGG CAG CGG CGG TGG ATG TA-3') in exon 9 (352) with a length bases 1389 bp. The results of the PCR amplification of the genomic DNA showed that 100% of the DNA bands amplified with primers specific to the length of the base 1389 bp exon 9 (352).