ISOLATION, CHEMICAL CHARACTERIZATION, AND BIOACTIVITY OF SECONDARY METABOLITES WITH POLAR CONSTITUENTS OF Petrossian alfiani SPONGES
| Main Author: | Usman, Hanapi |
|---|---|
| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
Consortium of Indonesian Biotechnology
, 2014
|
| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/10505 |
Daftar Isi:
- MAKALAH PRESENTATION
- Extraction and isolation of semi-polar and polar constituents from Petrossian Alfiani sponges in chloroform and ethyl acetate solvents has been performed. Petrossian Alfiani is an endemic species in the waters of Spermonde archipelago of South Sulawesi Indonesian. Five isolates (pure compounds) were resulted, two from the chloroform extract and three from the ethyl acetate extract. Both isolates from the chloroform extract were in the form of white crystals, namely I1 and I2. Spectroscopic analysis showed that the compound I1 is ??-Sitosterol. Four other isolates, respectively, I2 was identified as steroids, F3 as terpenoids, F6 and F7 as flavonoids. Bioactivity test for the level of toxicity was conducted on shrimp larvae Artemia salina leach. Compound I1 is very active with LC50 value of 1.47 ??g/mL. Compounds F3 shows the resistance activity to the C. albicans fungi.