Phytochemical screening and antibacterial activity of crude extracts from Jatropha curcas, Linn against histamine-forming bacteria
| Main Authors: | Setha, Beni, Laga, Amran, Mahendradatta, Meta, Firdaus |
|---|---|
| Format: | Article |
| Terbitan: |
Elixir Org. Chem
, 2014
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| Subjects: | |
| Online Access: |
http://repository.unhas.ac.id/handle/123456789/10096 |
Daftar Isi:
- Crude extract from Jatropha curcas L. active to against histamine-forming bacteria
- This study aims to analyze the phytochemical screening and antibacterial activity of leaves extract of Jatropha curcas against histamine forming bacteria, and determine of minimuminhibitory concentration. The extraction process is done by maceration method using hexane, chloroform, ethyl acetate and methanol. The extract was obtained continued by antibacterial activity test against histamine-forming bacteria. Concentration all extract of J. curcas is 1%, 10%, 20%, and 30% (w / v). Positive control using synthetic antibiotic ampicillin and negative controls used dimethyl sulfoxide (DMSO). The results of phytochemical screening showed that ethyl acetate and methanol extracts containing 5 secondary metabolites, while the hexane and chloroform containing only 2 secondary metabolites. Antibacterial activity test showed that the negative control and positive control do not provide inhibition against test bacteria. All types of extracts with concentrations of 1% to 30% (w / v) gave inhibition against test bacteria. The diameter of the inhibition of K. pneumoniae and C. perfringens for all jatropha leaf extract at a concentration of 1% (w / v) did not show any significant difference to the concentration of 10%. Lowest MIC (0.10% w / v) there at the ethyl acetate extract (E3) and methanol (E4) for the bacteria C. perfringens.