Encapsulation of Alkaline Phosphatase in Mesoporous Methyl-Silica Hybrid by Sol-Gel Process
| Main Author: | Sriyanti, Sriyanti |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
, 2017
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| Subjects: | |
| Online Access: |
https://ejournal.undip.ac.id/index.php/ksa/article/view/16716 https://ejournal.undip.ac.id/index.php/ksa/article/view/16716/12165 |
Daftar Isi:
- In recent years, the sol-gel technique has attracted increasing interest as a unique approach to immobilize biomolecules for bioanalitical applications as well as biochemical and biophysical studies. In this research, encapsulation of Alkaline Phosphatase (ALP) enzyme in mesoporous methyl-silica hybrid by sol-gel process has been carried out. Mesoporous methyl-silica hybrid has been synthesis by using tetraethylorthosilicate (TEOS) and methyltriethoxysilane (MTES) as precursor and poly(ethylene) glycol (PEG) as a polymer dopant. The preparation of methyl-silica hybrid was carried out at various mol ratios of precursors and PEG concentration. Encapsulation was emphasized by mixing sol solution and tris-HCl buffer containing ALP. The Surface Area Analyzer analysis data showed that the pore diameter and surface area of methyl-silica hybrid tended to increase as the PEG dopant concentration increases. Result showed that ALP encapsulated in the methyl-silica hybrids with PEG dopant gave enzymatic activity as 0.112 U/mg enzyme, higher than ALP encapsulated in copolymerization of TEOS with MTES or in silica from TEOS precursor only (0.098 U/mg enzyme and 0.069 U/mg enzyme, respectively). It was shown that copolymerization of TEOS with organosilane precursor and the entrapment of polymer additives into sol-gel processed materials are useful methods for modifying the internal environment and hence the activity of biomolecules entrapped in sol-gel derived biomaterials.