HIGH-THROUGHPUT IN VITRO CONSTRUCTION OF PROTEIN LIBRARY: A POTENTIAL TECHNOLOGY FOR CONTAINING AN EMERGING PANDEMIC AVIAN INFLUENZA
| Main Authors: | Ali, Muhamad, Yunita Sabrina, Yunita |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Subjects: | |
| Online Access: |
http://eprints.unram.ac.id/18242/1/9.%20SI-08%20High-throughput.pdf http://eprints.unram.ac.id/18242/ |
Daftar Isi:
- A highly pathogenic avian influenza A virus (subtype H5N1) that circulates in wild and domesticated poultry is the most serious human disease for decades. This virus is sporadically transmited from poultry to human and limited probable humanto-human transmission. Since the avian virus is RNA virus which reassort quickly, transmission to human is very fast and difficult to combat. Therefore, identifying a ultra-high-troughput technology to halt a pandemic is very urgent. In vitro construction of protein library using cell-free technology was found to be successful in rapid screening and generation of vaccine and monoclonal antibodies. This technology can by pass most of the biological process and allows itself to robotic automation for ultra-high-throughput screening and generation of suitable recombinant functional protein to combat highly spread and pathogenic H5N1 viruses.