Molasses as an Alternative Low-cost Medium for Production of Recombinant vp28-Antigen in Escherichia coli
| Main Authors: | Ali, Muhamad, N. Depamede, Sulaiman, Amin, Muhamad, Sukarne, Sukarne |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Subjects: | |
| Online Access: |
http://eprints.unram.ac.id/18240/1/7.%20SI-06%20Molases.pdf http://eprints.unram.ac.id/18240/ |
Daftar Isi:
- Previous studies reported that VP28, which is a capsid protein of White Spot Syndrome Virus (WSSV), showed to be a potential candidate for WSSV vaccine. However, the large-scale production of the protein is hampered by the availability and expensive cost of culture media. Thus, this study was aimed at finding alternative low-cost medium for growing bacterial hosts carrying plasmid for producing VP28 proteins fused with glutathione s-transferase (GST) enzyme which then indicated as GST.VP28 fusion protein. The result showed that 7.5% molasses seemed to have better growth than commercial media (LB). Other result also indicated that quality of GST.VP28 protein expressed by host in 7.5% molasses appeared to be good. Ass indicated by its solubility. This result suggested that molasses medium can be an alternative low-cost medium for large-scale production of GST.VP28 protein.