Isolation and Characterization of Excretory/Secretory Antigenic Proteins of Adult Fasciola gigantica Lombok Isolate
| Main Authors: | Ali, Muhamad, Made Sriasih, Sriasih, Sulaiman N. Depamede, Sulaiman |
|---|---|
| Format: | Proceeding PeerReviewed Book |
| Bahasa: | eng |
| Subjects: | |
| Online Access: |
http://eprints.unram.ac.id/18235/1/2.%20SI-01%20Isolation%20and%20caharcterization%20.pdf http://eprints.unram.ac.id/18235/ |
Daftar Isi:
- The objective of this study was to isolate excretory/secretory (ES) proteins of adult Fasciola gigantica local isolate (Lombok) and to determine its antigenic proteins using Western blotting. The ES components were isolated from live adult F. gigantica collected from liver of Fasciolosis positive Bali cattle slaughtered at the abattoir. Isolated ES were then characterized using 1-D gel electrophoresis and Western blotting. Gel electrophoresis analysis showed that the ES components of F. gigantica Lombok isolate consist of proteins with a molecular weight of 7 to 25 kDa. Western blotting analysis using sera of Fasciolosis positive Bali cattle (n = 2) exhibited several antigenic proteins with a molecular weight of 47.7, 25, 21.4 to 17.9, 16.4 and 10.5 to 8.05 kDa. Amongst the proteins detected, the 25 kDa was sharply reacted in both positive sample. This study suggests that the proteins recognised are an immunologically active component of the ES and antibodies to those proteins were present in naturally infected cattle. The results of this study are expected to support the Fasciolosis control program in cattle through a specific early detection and vaccination.