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DETEKSI MOLEKULER Mycobacterium tuberculosis DI DAHAK CARA POLYMERASE CHAIN REACTION

Main Authors: Notopuro, P. B., Nugraha, J., Notopuro, H.
Format: Article info application/pdf eJournal
Bahasa: eng
Terbitan: PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA , 2018
Subjects:
PCR
culture
IS6110 region primer
Online Access: http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944/670
ctrlnum article-944
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">DETEKSI MOLEKULER Mycobacterium tuberculosis DI DAHAK CARA POLYMERASE CHAIN REACTION</title><creator>Notopuro, P. B.</creator><creator>Nugraha, J.</creator><creator>Notopuro, H.</creator><subject lang="en-US">PCR, culture, IS6110 region primer</subject><description lang="en-US">tuberculosis is a chronic infectious disease which is found in developing and developed country. It is one of community healthproblems which become priority in national and international health programs. Microbiologic examination is used to establish thediagnosis of tuberculosis beside clinical examination and radiologic examination. Conventional microscopic and culture examinationhave many limitation ie: such as for example low sensitivity, specificity and need a lot of time. New Molecular technique gives morevalue in sensitivity, specificity and the time for examination. the aim of this study was to know the diagnostic value of PolymeraseChain Reaction for detection of Mycobacterium tuberculosis in sputum. the sputum was collected from twenty eight patients suspectedtuberculosis based on the clinical and radiological examination. the study was performed from September 2006 until July 2007. Wedid the conventional culture technique as a diagnostic gold standard and molecular technique to detect the Mycobacterium tuberculosisin the sputum. For molecular technique, we used Polymerase Chain Reaction (PCR) with a set of IS6110 region primer which is specificfor the Mycobacterium tuberculosis Complex. the sensitivity of PCR with IS6110 region primer is 100% (very high), specificity is 82.4%(high), positive predictive value is 89.7% and negative predictive value is 100%. there was statistically no significant difference betweenthe result of PCR and conventional culture method. Based on the result, the Polymerase Chain Reaction examination with primer IS6110region primer can be used as the screening tool for tuberculosis infection, while the clinician waits for culture result.</description><publisher lang="en-US">PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA</publisher><contributor lang="en-US"/><date>2018-03-16</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944</identifier><identifier>10.24293/ijcpml.v15i1.944</identifier><source lang="en-US">INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY; Vol 15, No 1 (2007); 16-21</source><source>2477-4685</source><source>0854-4263</source><source>10.24293/ijcpml.v15i1</source><language>eng</language><relation>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944/670</relation><rights lang="en-US">Copyright (c) 2018 INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc-nd/4.0</rights><recordID>article-944</recordID></dc>
language eng
format Journal:Article
Journal
Other:info:eu-repo/semantics/publishedVersion
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File:application/pdf
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Journal:eJournal
author Notopuro, P. B.
Nugraha, J.
Notopuro, H.
title DETEKSI MOLEKULER Mycobacterium tuberculosis DI DAHAK CARA POLYMERASE CHAIN REACTION
publisher PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
publishDate 2018
topic PCR
culture
IS6110 region primer
url http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/944/670
contents tuberculosis is a chronic infectious disease which is found in developing and developed country. It is one of community healthproblems which become priority in national and international health programs. Microbiologic examination is used to establish thediagnosis of tuberculosis beside clinical examination and radiologic examination. Conventional microscopic and culture examinationhave many limitation ie: such as for example low sensitivity, specificity and need a lot of time. New Molecular technique gives morevalue in sensitivity, specificity and the time for examination. the aim of this study was to know the diagnostic value of PolymeraseChain Reaction for detection of Mycobacterium tuberculosis in sputum. the sputum was collected from twenty eight patients suspectedtuberculosis based on the clinical and radiological examination. the study was performed from September 2006 until July 2007. Wedid the conventional culture technique as a diagnostic gold standard and molecular technique to detect the Mycobacterium tuberculosisin the sputum. For molecular technique, we used Polymerase Chain Reaction (PCR) with a set of IS6110 region primer which is specificfor the Mycobacterium tuberculosis Complex. the sensitivity of PCR with IS6110 region primer is 100% (very high), specificity is 82.4%(high), positive predictive value is 89.7% and negative predictive value is 100%. there was statistically no significant difference betweenthe result of PCR and conventional culture method. Based on the result, the Polymerase Chain Reaction examination with primer IS6110region primer can be used as the screening tool for tuberculosis infection, while the clinician waits for culture result.
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institution Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
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library Indonesian Journal of Clinical Pathology and Medical Laboratory
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collection Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML)
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subject_area Patologi Klinik
city KOTA SURABAYA
province JAWA TIMUR
repoId IOS5364
first_indexed 2018-04-01T07:53:02Z
last_indexed 2018-04-01T07:53:16Z
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