THE MORPHOLOGICAL FEATURES OF ERYTHROCYTES IN STORED PACKED RED CELLS
| Main Authors: | Kartini, .Dewi Sri, Muhiddin, Rahmawati, Arief, Mansyur |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
, 2017
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| Subjects: | |
| Online Access: |
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/718 http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/718/483 |
Daftar Isi:
- The morphology of erythrocyte Packed Red Cells (PRC) is subject to changes during storage at 2o–8oC. In maintaining theirviability, erythrocytes require adenosine triphosphate (ATP). The decrease in intracellular ATP level results in damaged membranelipid, accumulation of intracellular Sodium and Calcium, decreased intracellular potassium and water, cell dehydration, rigidity ofmembrane and changes in its shape from a disc into crenated red cells, spherocytes and bite cells. This study was aimed to observethe presentation of erythrocyte crenated cells, spherocytes and bite cells of stored PRC at day 3 (control), day 7, day 14 and day 21from the blood collection date of the blood bag. An observational study with cohort study approach was conducted in August 2015 atthe Blood Bank of Dr. Wahidin Sudirohusodo Hospital Makassar. The total sample was 30 blood bag that resulted in 120 slide bloodsmears. From 30 samples of blood bags comprising blood group A 26.6%, B 13.3%, AB 16.6% and O 43.3%, the increased percentagesof crenated cells, spherocytes and bite cells were observed at day 3, day 7, day 14 and day 21. Day 3 of storage was taken as control.Data were processed by Fiedman test and Wilcoxon test with significance level at p<0.001. There was an increase in the percentageof erythrocyte morphology change (crenated cells, spherocytes and bite cells) along with the storage time of packed red blood cells.The use of packed red blood cells was recommended not more than 21 days of storage.