COMPARISON RESULTS OF ANALYTICAL PROFILE INDEX AND DISC DIFFUSION ANTIMICROBIAL SUSCEPTIBILITY TEST TO TECHNICAL DEDICATED REASONABLE 300B METHOD
| Main Authors: | Sugiartha, IG, Sri Rejeki, IGAA Putri, Wardhani, Puspa, Semedi, Bambang Pujo |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
, 2017
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| Subjects: | |
| Online Access: |
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/701 http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/701/466 |
Daftar Isi:
- The bloodstream infection death rate is quite high, ranging from 20% to 50%. Causable pathogens could be demonstratedby blood cultures followed by antimicrobial susceptibility tests. The test could be performed in a manual, semiautomatic or fullyautomatic method. The manual method does not require large investment costs compared to automatic methods. This was anobservational cross sectional design study. Manual identification method used API and antimicrobial susceptibility tests used discdiffusion method of Kirby Bauer. Both methods were compared to semiautomatic TDR-300B method. A fully automatic VITEK 2method was used as the reference method for assessing the performance of manual and semiautomatic methods. Bacteria that causedbloodstream infections were mostly dominated by Gram-negative Escherichia coli and Klebsiella pneumonia. The accuracy of APIidentification method to the VITEK 2 was 87.87%, accuracy of TDR-300B identification method to VITEK 2 method was 90.9%. Theaccuracy results of manual Kirby Bauer disc diffusion antimicrobial susceptibility tests method compared to VITEK 2 method was84.64%. Accuracy of TDR-300B antimicrobial susceptibility tests method to VITEK 2 was 82.5%. The accuracy of API identificationmethod to TDR-300B was 84.84%.The accuracy of manual antimicrobial susceptibility test method to TDR-300B was 78.21%. Theresults of manual identification and antimicrobial sensitivity tests were not statistically significanly different with semiautomaticTDR-300B method. Manual identification and antimicrobial susceptibility test methods could be trusted, especially for financiallimited region or small number of examination.