DETECTION OF MYCOBACTERIUM TUBERCULOSIS WITH TB ANTIGEN RAPID TEST IN PULMONARY TUBERCULOSIS PATIENTS WITH FOUR TYPES OF SPUCTUM SAMPLE PREPARATION
| Main Authors: | Ilmiah, Miftahul, Sri Rejeki, IGAA Putri, Tambunan, Betty Agustina |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
, 2017
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| Subjects: | |
| Online Access: |
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/699 http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/699/464 |
Daftar Isi:
- Direct microscopic examination to diagnose TB has many limitations. TB Ag Rapid Test Device is an ICT examination methoddetecting TB-specific antigen secreted from the regions of difference (RD) of M.tuberculosis. Previous research indicated that tests cannot be always performed well. Samples were 30 positive sputum smears by direct microscopy sputum. Sputum samples underwent4 treatments: routine treatment according to the kit procedure; addition of 0.5 mL 2.5% NALC and whipped according to the kitprocedure; vortex treatment followed by centrifugation 10,000g temperature 4°C; addition of 0.5 mL 2.5% NALC, followed by vortexcentrifugation 10,000g temperature 4°C. The results of the antigen examination using TB Ag Rapid Test Device were as follows firstroutine treatment is 43.3% positive, second treatment had a high positive result (96.7%), third and fourth treatment had positiveresult 36.7% and 86.7%. Examination of Ag using vortex-centrifugation with a routine examination showed a compatibility of 86.6%(33.3% positive and 53.5% negative) with a Kappa value of 0.724 (p<0.0001). Treatment 2 (addition of 0.5 mL 2.5% NALC) withtreatment 4 (addition of 0.5 mL 2.5% NALC- vortex-centrifugation) demonstrated a compatibility of 90% (86.7% positive and 3.3%negative) with a Kappa value of 0.366 (p=0.010). The addition of 0.5 mL 2.5% NALC pretreatment can be used to increase the positiveoutcome of TB Ag Rapid Test Device for diagnosing pulmonary tuberculosis.