IMUNISASI PROTEIN ADHESIN 38-KDA MYCOBACTERIUM TUBERCULOSIS LEWAT RONGGA MULUT TERKAIT SEL T CD8+ DI PARU
| Main Authors: | Arthamin, Maimun Z, Gani, Agus A, Issiyah, Nurani, Santoso, Sanarto |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA
, 2016
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| Subjects: | |
| Online Access: |
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377 http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377/125 |
| ctrlnum |
article-377 |
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| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="en-US">IMUNISASI PROTEIN ADHESIN 38-KDA MYCOBACTERIUM TUBERCULOSIS LEWAT RONGGA MULUT TERKAIT SEL T CD8+ DI PARU</title><creator>Arthamin, Maimun Z</creator><creator>Gani, Agus A</creator><creator>Issiyah, Nurani</creator><creator>Santoso, Sanarto</creator><subject lang="en-US">38-kDa adhesin protein;mycobacterium tuberculosis;CD8+ T cells;lung;oral immunization</subject><description lang="en-US">The efficacy of Bacillus Calmette-Guerin (BCG), vaccine against tuberculosis (TB), varies widely, from 0 to 90%; and BCG mainly activates CD4+ T cells, but it fails to activate CD8+ T cells. From the previous study, 38-kDa protein is an adhesin protein. CD8+ T cells play the role in controlling Mycobacterium tuberculosis (M.tb) infection and contribute to the memory immunity. The objective of this study was to determine effect of oral immunization by 38-kDa adhesin protein of M.tb to increase the level of CD8+ T cells in the lung of BALB/c mice. This study used an experimental with post test control group design. The mice were divided into six groups (each group consist of 4 samples), where Group 1: were immunization orally with 100 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs. Followed by group 2: 100 μg 38-kDa adhesin protein of M.tb, group 3: 50 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs, and group 4: 50 μg 38-kDa adhesin protein of M.tb. Group 5: 12 μg ISCOMs. Group 6: Control. In this study was found increased level of CD8+ T cells in the lung of BALB/c mice after orally immunization with 38-kDa adhesin protein of M.tb. The highest level of CD8+ T cells was on group 1, p=0.000. Also there were found significant differences among the immunized groups, except group 2 and 3, as well as group 5 and 6 also. It can be concluded in this study that oral immunization with 38-kDa adhesin protein of M.tuberculosis could increase the level of CD8+ T cells in the lung of BALB/c mice.</description><publisher lang="en-US">PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA</publisher><contributor lang="en-US"/><date>2016-10-14</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Journal:Article</type><type>File:application/pdf</type><identifier>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377</identifier><identifier>10.24293/ijcpml.v18i3.377</identifier><source lang="en-US">INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY; Vol 18, No 3 (2012); 184–190</source><source>2477-4685</source><source>0854-4263</source><source>10.24293/ijcpml.v18i3</source><language>eng</language><relation>http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377/125</relation><rights lang="en-US">Copyright (c) 2016 INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY</rights><rights lang="en-US">http://creativecommons.org/licenses/by-nc-nd/4.0</rights><recordID>article-377</recordID></dc>
|
| language |
eng |
| format |
Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other File:application/pdf File Journal:eJournal |
| author |
Arthamin, Maimun Z Gani, Agus A Issiyah, Nurani Santoso, Sanarto |
| title |
IMUNISASI PROTEIN ADHESIN 38-KDA MYCOBACTERIUM TUBERCULOSIS LEWAT RONGGA MULUT TERKAIT SEL T CD8+ DI PARU |
| publisher |
PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA |
| publishDate |
2016 |
| topic |
38-kDa adhesin protein mycobacterium tuberculosis CD8+ T cells lung oral immunization |
| url |
http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377 http://www.indonesianjournalofclinicalpathology.or.id/index.php/patologi/article/view/377/125 |
| contents |
The efficacy of Bacillus Calmette-Guerin (BCG), vaccine against tuberculosis (TB), varies widely, from 0 to 90%; and BCG mainly activates CD4+ T cells, but it fails to activate CD8+ T cells. From the previous study, 38-kDa protein is an adhesin protein. CD8+ T cells play the role in controlling Mycobacterium tuberculosis (M.tb) infection and contribute to the memory immunity. The objective of this study was to determine effect of oral immunization by 38-kDa adhesin protein of M.tb to increase the level of CD8+ T cells in the lung of BALB/c mice. This study used an experimental with post test control group design. The mice were divided into six groups (each group consist of 4 samples), where Group 1: were immunization orally with 100 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs. Followed by group 2: 100 μg 38-kDa adhesin protein of M.tb, group 3: 50 μg 38-kDa adhesin protein of M.tb and 12 μg ISCOMs, and group 4: 50 μg 38-kDa adhesin protein of M.tb. Group 5: 12 μg ISCOMs. Group 6: Control. In this study was found increased level of CD8+ T cells in the lung of BALB/c mice after orally immunization with 38-kDa adhesin protein of M.tb. The highest level of CD8+ T cells was on group 1, p=0.000. Also there were found significant differences among the immunized groups, except group 2 and 3, as well as group 5 and 6 also. It can be concluded in this study that oral immunization with 38-kDa adhesin protein of M.tuberculosis could increase the level of CD8+ T cells in the lung of BALB/c mice. |
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IOS5364.article-377 |
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Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML) |
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1807 |
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library:special library |
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Indonesian Journal of Clinical Pathology and Medical Laboratory |
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1493 |
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Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCP & ML) |
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5364 |
| subject_area |
Patologi Klinik |
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KOTA SURABAYA |
| province |
JAWA TIMUR |
| repoId |
IOS5364 |
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2018-03-14T22:04:32Z |
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2018-03-14T22:04:55Z |
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