OPTIMASI DAN KARAKTERISASI a-AMILASE DARI ISOLAT AKTINOMISETES YANG BERASAL DARI KALIMANTAN TIMUR
| Main Author: | Soeka, Yati Sudaryati |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Research Center for Biology-Indonesian Institute of Sciences
, 2015
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| Subjects: | |
| Online Access: |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/751 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/751/523 |
Daftar Isi:
- Forty-one actinomycetes isolates from East Kalimantan held in Microbiology Division Collection-UPI, and their ability to produce a-amylase has been assessed. Those 41 number of actinomycetes isolates performed amylolytic activity as shown by clear zone areal after being poured with iodium solution. The bacteria produced high a-amylase when was grown in media containing starch soluble 2% the a-amylase activity in media containing 8.24 U/ml. The isolate (number 7) was the most active compared to another (number 100) and it was identified as Nocardia; the activity of this enzyme obtained was 12.93 U/ml (one unit activity is defined as mol of glucose produced per ml per minute). The maximum temperature for enzyme reaction was 40°C, optimum pH was pH 7.5 the a-amylase activity were 15.76 U/ml and 31.11 U/ml, respectively. From kinetic characterization study, it was found that enzyme showed Km and Vmax value of 7.62 % (b/v) and 71.10- umol/ml/minute respectively at condition of temperature 40°C, pH 7.5 and incubation time 10 minute.