PENGARUH 2,4-D DAN BA TERHADAPINDUKSIKALUS EMBRIOGENIK PADA KULTUR MERISTEM JAHE {Zingiber officinale Rose.
| Main Authors: | Sitinjak, Rama Riana, Rostiana, Otih, Karyono, Karyono, Supriatun, Titin |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Research Center for Biology-Indonesian Institute of Sciences
, 2016
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| Subjects: | |
| Online Access: |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2037 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/2037/1916 |
Daftar Isi:
- The aim of this study was to evaluate the effects of 2,4-dichlorophenoxyacetic acid (2,4-D) and N -benzyladenin (BA) to embryogenic callus induction of meristem culture in ginger {Zingiber officinale Rose). Completely Randomixed Design was applied using factorial pattern, replicated 3 times. Two factors were assigned i.e: 2,4-D (1.0, 2.0 and 3.0 mg/L) and BA (0.0, 1.0, 3.0 and 5.0 mg/L). Result showed that 2,4-D and BA significantly affected the embryogenic callus induction. The highest embryogenic callus (93.33%) was achieved on MS (Murashige & Skoog) containing combination of 1.0 mg/L 2,4-D and 3.0 mg/L BA, after 8weeks incubation.Histological evaluation informed that the incubation of embryogenic calli started from cortex cells adjacent the meristem vascular tissues.