PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN [Vegetative Propagation of Chrysant by Tissue Culture Technique]
| Main Authors: | Dwimahyani, Ita, Gandanegara, S |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Research Center for Biology-Indonesian Institute of Sciences
, 2016
|
| Subjects: | |
| Online Access: |
http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127 http://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/1127/1002 |
Daftar Isi:
- Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.