Tampilan Petugas: REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)

REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)

Main Authors:	Sianturi, Ratna Damayanti, Supriyanto, Supriyanto, Wulandari, Arum Sekar, Subandy, Benny
Other Authors:	Balai Penelitian Teknologi Perbenihan Tanaman Hutan
Format:	Article info application/pdf eJournal
Bahasa:	ind
Terbitan:	Pusat Penelitian dan Pengembangan Hutan , 2017
Subjects:	Fagraea fragrans tissue culture medium organogenesis kultur jaringan media
Online Access:	http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931/2161

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fullrecord	<?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)</title><creator>Sianturi, Ratna Damayanti</creator><creator>Supriyanto, Supriyanto</creator><creator>Wulandari, Arum Sekar</creator><creator>Subandy, Benny</creator><subject lang="id-ID">Fagraea fragrans; tissue culture; medium; organogenesis; Fagraea fragrans; kultur jaringan; media; organogenesis</subject><description lang="id-ID"> ABSTRACTembesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program,and it can be achieved by tissue culture. The objective of the research was to find out the best modificationmedium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitiousshoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitiousshoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The resultsshowed (1) addition of BAP 1.5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves;(2) addition of BAP 0.1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3).clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant inacclimatization stage.Keywords : Fagraea fragrans, tissue culture, medium, organogenesisABSTRAKTembesu (Fagraea fragrans Roxb.) merupakan jenis pohon yang mampu tumbuh di hutan rawa gambut dan bernilai ekonomi tinggi. Untuk mendukung program rehabilitasi lahan gambut, maka diperlukan bibit yangberkualitas, jumlah yang cukup dan tepat waktu. Upaya yang diperlukan dalam menyediakan bibit tersebutadalah perbanyakan bibit melalui teknik kultur jaringan. Tujuan penelitian adalah mendapatkan komposisimedia tumbuh MS dan zat pengatur tumbuh yang tepat dalam pembentukan dan perbanyakan tunas adventifdari daun tembesu. Metode penelitian terdiri dari persiapan bahan tanaman, induksi tunas adventif,perbanyakan tunas adventif, elongasi, pengakaran dan aklimatisasi. Penelitian menunjukkan bahwa hasilyang terbaik yaitu (1) untuk menginduksi tunas adventif diperlukan media MS (80 mmol N) denganpenambahan BAP 1,5 ppm; (2) untuk perbanyakan tunas adventif diperlukan media MS (60 mmol N) denganpenambahan 0,1 ppm BAP; (3) pada tahap elongasi dan pengakaran lebih baik menggunakan tunas adventifdari klon 2; dan (4) pada tahap aklimatisasi lebih baik menggunakan tunas adventif klon 4.Kata kunci : Fagraea fragrans, kultur jaringan, media, organogenesis </description><publisher lang="id-ID">Pusat Penelitian dan Pengembangan Hutan</publisher><contributor lang="id-ID">Balai Penelitian Teknologi Perbenihan Tanaman Hutan</contributor><date>2017-06-22</date><type>Journal:Article</type><type>Other:info:eu-repo/semantics/publishedVersion</type><type>Other:</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931</identifier><identifier>10.20886/jpht.2017.14.1.1-17</identifier><source lang="en-US">Jurnal Penelitian Hutan Tanaman; Vol. 14, No. 1 (2017): JURNAL PENELITIAN HUTAN TANAMAN; 1-17</source><source lang="id-ID">Jurnal Penelitian Hutan Tanaman; Vol. 14, No. 1 (2017): JURNAL PENELITIAN HUTAN TANAMAN; 1-17</source><source>2442-8930</source><source>1829-6327</source><language>ind</language><relation>http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931/2161</relation><rights lang="id-ID">##submission.copyrightStatement##</rights><rights lang="id-ID">http://creativecommons.org/licenses/by-nc/4.0</rights><recordID>--ejournal.forda-mof.org-ejournal-litbang-index.php-index-oai?verb=ListSets:article-1931</recordID></dc>
language	ind
format	Journal:Article Journal Other:info:eu-repo/semantics/publishedVersion Other Other: File:application/pdf File Journal:eJournal
author	Sianturi, Ratna Damayanti Supriyanto, Supriyanto Wulandari, Arum Sekar Subandy, Benny
author2	Balai Penelitian Teknologi Perbenihan Tanaman Hutan
title	REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)
publisher	Pusat Penelitian dan Pengembangan Hutan
publishDate	2017
topic	Fagraea fragrans tissue culture medium organogenesis kultur jaringan media
url	http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931 http://ejournal.forda-mof.org/ejournal-litbang/index.php/JPHT/article/view/1931/2161
contents	ABSTRACTembesu (Fagraea fragrans Roxb.) is one of native tree species in peatland forest and has high economical values. Sufficient amount in time of qualified seedlings is needed to support peatland rehabilitation program,and it can be achieved by tissue culture. The objective of the research was to find out the best modificationmedium of MS based on concentration of nitrogen and BAP for induction and multiplication of adventitiousshoot from leaves. The protocol of tissue culture consisted of preparation of plant material, adventitiousshoots induction, shoots multiplication, shoots elongation, rooting and seedling acclimatization. The resultsshowed (1) addition of BAP 1.5 ppm on MS medium (80 mmol N) induced adventitious shoots from leaves;(2) addition of BAP 0.1 ppm on MS medium (80 mmol N) stimulated the highest multiplication of shoots; (3).clone 2 was the best explant on elongation and rooting stage; (4). clone 4 was the best explant inacclimatization stage.Keywords : Fagraea fragrans, tissue culture, medium, organogenesisABSTRAKTembesu (Fagraea fragrans Roxb.) merupakan jenis pohon yang mampu tumbuh di hutan rawa gambut dan bernilai ekonomi tinggi. Untuk mendukung program rehabilitasi lahan gambut, maka diperlukan bibit yangberkualitas, jumlah yang cukup dan tepat waktu. Upaya yang diperlukan dalam menyediakan bibit tersebutadalah perbanyakan bibit melalui teknik kultur jaringan. Tujuan penelitian adalah mendapatkan komposisimedia tumbuh MS dan zat pengatur tumbuh yang tepat dalam pembentukan dan perbanyakan tunas adventifdari daun tembesu. Metode penelitian terdiri dari persiapan bahan tanaman, induksi tunas adventif,perbanyakan tunas adventif, elongasi, pengakaran dan aklimatisasi. Penelitian menunjukkan bahwa hasilyang terbaik yaitu (1) untuk menginduksi tunas adventif diperlukan media MS (80 mmol N) denganpenambahan BAP 1,5 ppm; (2) untuk perbanyakan tunas adventif diperlukan media MS (60 mmol N) denganpenambahan 0,1 ppm BAP; (3) pada tahap elongasi dan pengakaran lebih baik menggunakan tunas adventifdari klon 2; dan (4) pada tahap aklimatisasi lebih baik menggunakan tunas adventif klon 4.Kata kunci : Fagraea fragrans, kultur jaringan, media, organogenesis
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REGENERASI TUNAS ADVENTIF DARI EKSPLAN DAUN TEMBESU (Fagraea fragrans Roxb.) MELALUI TEKNIK KULTUR JARINGAN (Regeneration of Adventitious Shoots From Leaf Explant of Tembesu (Fagraea fragrans Roxb.) by tissue culture)

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