Deteksi Mycobacterium tuberculosis dengan Teknik PCR pada Cairan Efusi Pleura Penderita Tuberkulosis Paru
| Main Authors: | Jasaputra, Diana Krisanti; Universitas Kristen Maranatha, Widjaja, Jahja Teguh; Universitas Kristen Maranatha, Wargasetia, Teresa Liliana; Universitas Kristen Maranatha, Makangiras, Iryanthy; Universitas Kristen Maranatha |
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| Format: | application/octetstream eJournal |
| Bahasa: | eng |
| Terbitan: |
Universitas Kristen Maranatha
, 2010
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| Online Access: |
http://majour.maranatha.edu/index.php/jurnal-kedokteran/article/view/102 |
Daftar Isi:
- Tuberculosis pleural effusion is a manifestation of extrapulmonal tuberculosis, which is rampant in various countries in the world, including Indonesia. Nowadays one-third of the world population has been infected by tuberculosis. Every year there are approximately 8 million new tuberculosis patients throughout the world and nearly 3 million people die of this disease every year. Pathogenesis of tuberculosis pleural effusion is a pleural hypersensitivity reaction accompanied by a small number of Mycobacterium tuberculosis. The objective of this research was to detect Mycobacterium tuberculosis in pleural effusion liquid by means of PCR technique to establish diagnosis of tuberculosis pleural effusion. On this research, detection of Mycobacterium tuberculosis by PCR technique was compared with microscopic examination by means of Ziehl-Neelsen (ZN) method. The research result showed that 9 out of 11 samples were positive by using PCR technique whereas with ZN method all samples showed negative result. The conclusion of this research was that Mycobacterium tuberculosis in small numbers could be detected in pleural effusion liquid by means of PCR technique so that diagnosis could certainly be established.