Ekspresi dan Aktivasi Reseptor Glukokortikoid serta Ekspresi Heat Shock Protein- 90 pada Sel Limfosit Penderita Sindrom Nefrotik Idiopatik
| Main Authors: | Khairiyadi, Khairiyadi, Subandiyah, Krisni, Fitri, Loeki Enggar |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran Universitas Brawijaya
, 2013
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| Subjects: | |
| Online Access: |
https://jkb.ub.ac.id/index.php/jkb/article/view/314 https://jkb.ub.ac.id/index.php/jkb/article/view/314/301 |
Daftar Isi:
- Glucocorticoid receptors (GR) have an important role in the resistance of steroid therapy . Previous studies showed significant difference activation and expression of GR between Steroid Resistant Nephrotic Syndrome (SRNS) and Steroid Sensitive Nephrotic Syndrome (SSNS), however the other studies showed contrastive result. Previous studies also revealed Hsp-90 had an important role in GR activation. The aim of this study was to determine the differences expression and activation of GR and also Hsp-90 expression in lymphocytes cytoplasm and nucleus between SRNS and SSNS patients. A Cross-sectional study was conducted to 40 children (20 SRNS and 20 SSNS patients). Double stainning immunocytochemistry of lymphocytes was performed. The GR expression and activation were determined by counting amount of lymphocytes that express GR and Hsp-90 among 200 under light microscope with 1000x magnification. The differences of activation and activation of GR and Hsp-90 in cytoplasm and nucleus were analyzed with independent t-test and the correlation was analyzed with Pearson correlation.