Sterculia foetida leaf fraction against matrix metalloproteinase-9 protein and 4T1 breast cancer cells: In-vitro and in-silico studies
| Main Author: | R. Rollando, - |
|---|---|
| Format: | Article NonPeerReviewed |
| Online Access: |
http://repository.ub.ac.id/187150/ https://doi.org/10.26538/tjnpr/v5i1.15 |
Daftar Isi:
- Sterculia foetida leaf extract has been shown to have cytotoxic activity. Matrix metalloproteinase-9 (MMP-9) has an important role in pathophysiological functions. Inhibition of MMP9 is an important therapeutic approach for combating cancer. This study was conducted to determine the most active fraction of S. foetida as anti-breast cancer agent with hemopexin-like domain of MMP-9 (PEX9) as the selective protein target and 4T1 cells line as metastatic breast cancer cell. The leaves S. foetida was extracted using 80% methanol and was fractionated into fractions of n-hexane, chloroform, ethyl acetate, n-butanol, and insoluble n-butanol with liquid-liquid partition. In vitro screening against MMP-9 was performed using FRET-based assay and cytotoxic tests were performed using the MTT assay. Identification of compounds in the most active fraction using GC-MS. The docking to PEX9 was run using AutoDock Vina embedded in PyRx program. The n-hexane fraction was the most active fraction to inhibit MMP-9 with an IC50 of 19.67 μg/mL and inhibit the growth of 4T1 cells with an IC50 of 34.65 μg/mL. NNGH was used as positive control for the in-vitro and in-silico studies. The GC-MS results of the n-hexane fraction showed that there were 23 compounds, and they had binding affinity score of -8.9 to -4.9 kcal/mol towards PEX9. It can be concluded that S. foetida leaf has the potential to be developed for therapeutic use, especially for breast cancer therapy.