Integritas Membran Spermatozoa Persilangan Kambing Boerawa Dalam Pengencer Andromed® Dengan Penambahan Ekstrak Bawang Merah (Allium Cepa) Selama Penyimpanan Suhu Dingin (4-50c)
| Main Author: | Wara, PututBayu |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.ub.ac.id/137669/ |
Daftar Isi:
- Artificial Insemination needs a good quality of semen, on the other side, goat semen was easily damaged during cryoperservation in cold temperature storage. This condition was caused by the metabolism inside membrane plasma cell of the semen that produced Reactive Oxygen Species (ROS), which caused decreased the quality of the semen. This researched aims was to determined the dose of onion extracts in a diluent AndroMed®: 1%, 2%, and 3%. Goat semen obtained from the buck once a week using an artificial vagina. The results of this researched was the mean percentage of motility, viability and, membran integrity of boerawa crossbred semen in AndroMed® diluent with suplementation of onion extract 1%, 2%, and 3% were significantly different (P<0,05) in motility and viability, but gave high significantly different (P<0,01) to membran integrity. The best results are obtained adding onion extract on P2 is equal to 2%, obtained motility to 63,75 ± 5,62% on 1st hour, 56,25 ± 5,62%, on 2nd hour, and 50,00 ± 7,30% on 3rd hour, the viability obtained of 91,11 ± 2,85% on 1st hour, 80,07 ± 4,64% on 2nd hour, 76,96 ± 5,28% on 3rd hour, and the membran integrity on 1st hour 85,81 ± 1,47%, 76,67 ± 1,40% on 2nd, and 67,04 ± 2,12% on 3rd hour. The conclusion was the suplementation of onion extracts in AndroMed® diluent could protect spermatozoa to inhibit the development of ROS so semen quality could be maintained in cold temperature storage (motility, viability, and membrane integrity) that is suitable being used in the artificial insemination of goat