ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER (THE ISOLATED AND CHARACTERISTIC LACTIC ACID BACTERIA IN BROILER’S INTESTINE)
| Main Authors: | Sari, Meisji Liana, Abrar, Arfan, ., Merint |
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| Format: | Article PeerReviewed application/pdf |
| Terbitan: |
Jurusan Peternakan Fakultas Pertanian Universitas Syiah Kuala Banda Aceh
, 2013
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| Subjects: | |
| Online Access: |
http://eprints.unsri.ac.id/6578/1/Jurnal_Agripet_V.13_No.1_April_2013_Hal_43%2D48.pdf http://eprints.unsri.ac.id/6578/ |
Daftar Isi:
- The objective of the research was to avaluate and characterisize of a Lactid Acid Bacteria isolated frombroiler’s small intestine. The research was done by three steps : (1) Isolated Lactid Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria.The isolation of lactid acid bacteria was proceed by clone from broiler’s small intestine with MRS Broth for 24 hours, thinned until 10 ̄ 9with serial number, invested in MRS jell, purify by streak plate method and storage the lactid acid bacteria in an MRS angle jell. Gram colourize was done with cristal violet liquid, Iodium, Alkohol 95% and Safranin, and also microscopic observation was done by electrone microscope with 40 zooming. Inhibitory test with phatogen bacteria was evaluated with Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 dan 48 hours incubation period. The isolation result showed two sample of isolate (A1 dan A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate A1 the colonies had coccus and white colour. The isolate of A2 had sarcinar. After that, gram colouring was done and microscopic observation were identified into gram-negatife bacteria became bacill and bacill cocco.inhibitory test with phatogen bacteria showed that itr produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactbacillus sp as the genera of Lactid Acid Bacteria.